New research is revealing how red blood cells are made and how the body regulates the amount of haemoglobin that is packaged in red blood cells at any time. Genomic analysis techniques have doubled the number of genetic regions that are likely to be involved in red blood cell formation and subsequent study using fruit flies has given insights into what these regions do.
Haemoglobin is the protein which captures oxygen from the lungs for transport and delivery to tissues. It colours blood cells red and each day hundreds of millions of fresh red blood cells have to be formed by blood stem cells to replace the ones which come to the end of their life cycle. Anaemia, one of the most common disorders for which people visit their surgery, ensues if the production of new red blood cells is insufficient or their lifespan is shortened. The new genetic information is laying the foundations for future studies into the roots of anaemia by uncovering new biological pathways and mechanisms involved in controlling the size and number of red blood cells and the levels of haemoglobin.
The researchers used genome-wide association studies to identify genetic regions that appeared to influence the formation of red blood cells and their haemoglobin content.
"We studied the genetic influences behind six different physical parameters of red blood cells that reflect the volume and number of red blood cells and the levels of haemoglobin," says Dr John Chambers, lead author from Imperial College, London. "Our initial genetic association study looked into the genomes of 135,367 people and identified 75 genetic regions that directly influence these different traits of red blood cells. More than half – 43 – of these discoveries are new in people."
The team then closely examined using computational biology approaches the 75 genetic regions and the more than 3,000 genes responsible for protein production lie close to these regions. They prioritised 121 'candidate' genes or genes that are likely to regulate a trait in red blood cells from this list and investigated their function using information on model systems like from public databases as well as newly-generated data for fruit fly.
"Our work shows how model systems like fruit fly and mice can be used to provide insights into human genetics," says Professor Willem Ouwehand, lead author from the University of Cambridge and NHS Blood and Transplant. "We searched through a Mouse Genome database and found that 29 of our 121 candidate genes are linked to red blood cell formation in mice.
"These previous studies revealed that - when the function of these genes was switched off- the mice frequently developed reduced numbers of red blood cells, and anaemia. These observations made in mice make it highly likely that the remaining candidate genes, about which there is no knowledge yet, are also important regulators of red blood cell formation in people."
To investigate further, the team then reduced or 'silenced' the activity of the candidate genes in fruit flies. Although fruit flies do not have red blood cells, they share some of the gene functions leading to the formation of blood elements. These studies confirmed that sets of genes involved in controlling human red blood cell traits in people were also important for the formation of blood cells in fly.
"These results support the view that genetic association studies identify sets of genes that are conserved in evolution across a wide range of species," says Dr Nicole Soranzo, lead author from the Wellcome Trust Sanger Institute. "This is exciting because it means that we can obtain extensive new insights into the genetics and biological pathway of human health by studying model organisms.
"Although the underlying mechanisms for the majority of genes we've identified still need to be elucidated, our research is opening many doors for future studies on the generation of red blood cells for clinical use in the laboratory and may also provide insights which may lead to improvements in the treatment of patients with inherited anaemias."
Pim van der Harst, Weihua Zhang, Irene Mateo Leach et al (2012). '75 genetic loci influencing the human red blood cell'
Published online in Nature 05 December. DOI: 10.1038/nature11677
Wellcome Trust Sanger Institute: http://www.sanger.ac.uk
This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.
The brain has a weak spot for Alzheimer's disease and schizophrenia, according to experts who have pinpointed the region using scans.
Scientists in California have captured the elusive anglerfish on film in its natural habitat, 1,900 feet below sea level
Researchers were shocked to discover multiple instances of seal-on-penguin rape
The tiny shrimp survive without sunlight and crawl within an inch of boiling hot waters
Birds are one of the most widely studied forms of life on the planet. And, there are still new species out there to discover — as one young researcher found recently in a forest in Indonesia.
About once a year, Florida harvester ants dig new nests, a mystery entomologists are eager to get to the bottom of.
The finding that male homosexuality has a strong genetic component should be a boon for gay rights – but it could backfire
Alan Turing, the man who pioneered computing, also forced the world to question what it means to be human
During sleep, the brain locks in existing memories and can even form new ones. Scientists say they are starting to understand how that happens. A midnight snack may interfere.
They walk among us. Natural experiments, living ordinary lives, unaware that their genes may hold the clue to the next superdrug.