A new model of the how the protein coat (capsid) of viruses assembles, published in BioMed Central's open access journal BMC Biophysics, shows that the construction of intermediate structures prior to final capsid production (hierarchical assembly) can be more efficient than constructing the capsid protein by protein (direct assembly). The capsid enveloping a virus is essential for protection and propagation of the viral genome. Many viruses have evolved a self-assembly method which is so successful that the viral capsid can self assemble even when removed from its host cell.
The construction of large protein structures has been observed experimentally but the mechanism behind this is not well understood. Even the 'simple' icosahedral protein coat of the T1 virus requires integration of 60 protein components. Computational models of the physical interactions of component proteins are used to investigate the dynamics and physical constraints that regulate whether the components assemble correctly.
Using computer simulations a team from the Institute for Theoretical Physics and the Center for Quantitative Biology (BioQuant), University of Heidelberg, has compared direct and hierarchical assembly methods for T1 and T3 viruses. The team led by Ulrich S Schwarz, realised that direct assembly often led to the formation of unfavorable intermediates, especially when the dissociation rate was low, which hindered further assembly, causing the process to stall. In contrast, for many conditions hierarchical assembly was more reliable, especially if the bonds involved had a low dissociation rate.
Discussing the practical applications of these results, Dr Schwarz commented, "Hierarchical assembly has not been systematically investigated before. Theoretical models and computer simulations, like ours, can be used to understand the mechanism behind assembly of complex viruses and give an indication of how other large protein complexes assemble."
He continued, " With our computer simulations, we are now in a position to investigate systems which are too large to be studied by molecular resolution. This rational approach might have many applications not only in biomedicine, but also in materials science, where many researchers strive to learn from nature how to assembly complex structures."
BioMed Central: http://www.biomedcentral.com
This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.
Researchers were surprised by what they found when they sandwiched a drop of water between two layers of an unusual two-dimensional material called graphene.
Scientists at Cern are suggesting they could soon detect miniature black holes, proving the existence of parallel universes and disproving the big bang theory of the creation of the universe.
The Curiosity rover makes a detection of nitrogen compounds which provide further evidence that ancient Mars would have been a habitable world.
Wild animals can predict earthquakes several weeks before they strike, and motion-activated cameras that track their movements could be adopted in quake-prone countries as an affordable early warning system, scientists said on Tuesday.
GENEVA (Reuters) - Scientists at Europe's CERN research center have had to postpone the imminent relaunch of their refitted 'Big Bang' machine, the Large Hadron Collider, because of a short-circuit in the wiring of one of the vital magnets.
Images taken by NASA's Dawn spacecraft show that a mysterious bright spot on dwarf planet Ceres could be a plume of water spurting from a deep, icy crater
Using seismic vibrations from earthquakes around the world, they are figuring out what Earth looks like below the surface
In honor of a very special Pi Day, enjoy this map that explores the human-made and natural structures that come closest to a perfect circle
The moon has a more complex history than previously thought with at least nine subsurface layers, results from ground-penetrating radar aboard China’s Yutu lunar rover shows, scientists said on Thursday.
Scientists at the CERN physics research center said on Thursday the mystery dark matter that makes up 96 percent of the stuff of the universe will be a prime target for their souped-up Large Hadron Collider (LHC) in the coming years.