A new model of the how the protein coat (capsid) of viruses assembles, published in BioMed Central's open access journal BMC Biophysics, shows that the construction of intermediate structures prior to final capsid production (hierarchical assembly) can be more efficient than constructing the capsid protein by protein (direct assembly). The capsid enveloping a virus is essential for protection and propagation of the viral genome. Many viruses have evolved a self-assembly method which is so successful that the viral capsid can self assemble even when removed from its host cell.
The construction of large protein structures has been observed experimentally but the mechanism behind this is not well understood. Even the 'simple' icosahedral protein coat of the T1 virus requires integration of 60 protein components. Computational models of the physical interactions of component proteins are used to investigate the dynamics and physical constraints that regulate whether the components assemble correctly.
Using computer simulations a team from the Institute for Theoretical Physics and the Center for Quantitative Biology (BioQuant), University of Heidelberg, has compared direct and hierarchical assembly methods for T1 and T3 viruses. The team led by Ulrich S Schwarz, realised that direct assembly often led to the formation of unfavorable intermediates, especially when the dissociation rate was low, which hindered further assembly, causing the process to stall. In contrast, for many conditions hierarchical assembly was more reliable, especially if the bonds involved had a low dissociation rate.
Discussing the practical applications of these results, Dr Schwarz commented, "Hierarchical assembly has not been systematically investigated before. Theoretical models and computer simulations, like ours, can be used to understand the mechanism behind assembly of complex viruses and give an indication of how other large protein complexes assemble."
He continued, " With our computer simulations, we are now in a position to investigate systems which are too large to be studied by molecular resolution. This rational approach might have many applications not only in biomedicine, but also in materials science, where many researchers strive to learn from nature how to assembly complex structures."
BioMed Central: http://www.biomedcentral.com
This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.
Dutch biologist Ingrid van der Meer often meets with disbelief when she talks about her work on dandelions and how it could secure the future of road transport.
Pretend for a minute that it’s 1875 and you’re a mining engineer whose job it is to figure out how much gold is in them thar hills. Get it wrong, and your company is going to waste a lot of time and money hunting for gold that’s not there—or worse yet, miss out on the mother lode
It can only switch from black to transparent and back again, but that's a start
What happens when you add folds to materials that are only a few atoms thick? Several scientists set out to find the answer — and discovered that these nano-wrinkles can be quite useful.
A battery tattoo powered by perspiration has been unveiled by chemists in California.
Huge earthquakes could be on the way to Chile, after the major quake in April this year failed to relieve seismic stresses that have built up for 150 years
The Swedish government is looking at building a nuclear reactor purely for research, although a decision will not be taken until after a general election in September, Swedish daily Dagens Industri reported.
A new movie turns the physicist into a romantic lead. But how will it handle the not-so-wonderful parts of his marriage? Truthaholics want to know.
Iranian mathematician Prof Maryam Mirzakhani becomes the first woman to win a Fields Medal at a ceremony in Seoul, South Korea.
The urine test employers typically use to detect marijuana picks up cannabis smoked or swallowed days or weeks earlier. Should firms be allowed to fire workers who legally use marijuana at home?