A research team of scientists from EMBL Grenoble and the IGBMC in Strasbourg, France, have, for the first time, described in molecular detail the architecture of the central scaffold of TFIID: the human protein complex essential for transcription from DNA to mRNA. The study, published today in Nature, opens new perspectives in the study of transcription and of the structure and mechanism of other large multi-protein assemblies involved in gene regulation.
By controlling the transcription of DNA into messenger RNA, TFIID forms the cornerstone of the machinery that controls gene expression in our cells. Despite its crucial role, very little was known about its architecture. TFIID is present at very low levels in cells, and it is a very large protein complex made of 20 subunits: this combination largely prevented previous attempts to purify it and decipher its structure and function in molecular detail. Even the most advanced methods for recombinant protein production met their limits when trying to produce its various subunits in the right proportions.
The solution to this bottleneck came from studying the strategy certain viruses, such as Coronaviruses, use when they replicate: they produce very long protein chains that are then divided into individual proteins. Mimicking this technique led to highly abundant and correctly assembled complexes of the core scaffold of TFIID (comprising 10 subunits), which could be purified and analysed at high resolution by combining electron microscopy and data from X-ray crystallography.
"We know now in some detail what the core of TFIID looks like, and what happens when further subunits are bound. We believe that we have opened the door to determining the architecture of the entire human TFIID complex in the near future, and likewise of other large multiprotein assemblies involved in gene regulation, and to explain their roles in catalysing biological function," concludes Imre Berger, coordinator of the study at EMBL.
European Molecular Biology Laboratory: http://www.embl.org
This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.
It may not sound like the most useful of scientific endeavours, but the methods used to turn a hard-boiled egg back into its liquid state could bring major benefits to areas as diverse as cheese-making and cancer research
The science behind how water releases the funk from all the yeasts and bacteria hiding in your dog's fur.
The oldest rocky planets yet are 11.2 billion years old, just a little younger than the universe - meaning the galaxy made an early start on planet building
It's usually only possible to see the spot where a laser lands rather than its path, but now an ultrafast camera has caught those photons mid-flight
A team of Indian physicists has made a mathematical model that purports to explain why ants don't have traffic jams. NPR's Joe Palca explains as part of his series, Joe's Big Idea.
On a fall morning in 2009, a team of three young physicists huddled around a computer screen in a small office overlooking Broadway in New York. They were dressed for success—even the graduate student’s shirt had buttons—and a bottle of champagne was at the ready.
Light is the fastest thing around, but shaped beams travel slower than expected, even in a vacuum. No need to throw your physics textbooks out yet though
A new molten salt nuclear reactor design could make nuclear power safer and more economical.A new take on an old reactor design could make nuclear power cleaner and safer, and therefore more competitive with fossil fuels.
Forensic scientists can find crime-solving evidence in the tiniest details, such as the insects that arrive at the scene to feed on the decomposing corpse.
It may surprise you to learn that some processed foods made from GMOs — say, canola oil, for example — don't actually contain any genetically modified DNA or proteins.