Halfway between bacteria and tree
How the protein transport machinery in the chloroplasts of higher plants developed Moss Physcomitrella patens is an evolutionary intermediate stage
Together with colleagues from Sweden, RUB researchers have studied how the protein transport system of bacteria developed over time to form the system in the chloroplasts of higher plants. They explored the so-called signal recognition particles (SRP) and their receptors. Bioinformatic and biochemical analyses revealed that the moss Physcomitrella patens has evolutionarily old and new components of the SRP system, and thus represents an intermediate stage in the development from the bacterial transport system to the chloroplast system in higher plants. The international team led by Prof. Dr. Danja Schünemann and Dr. Chantal Träger from the Working Group Molecular Biology of Plant Organelles at the Ruhr-Universität reported in the journal The Plant Cell.
The SRP system guides new proteins to their place of work
In the cell fluid, a special transport machinery conveys proteins from their origin to their place of work, for example in the cell membrane. The decisive factor is the so-called SRP system. It binds itself to the protein to be transported, travels with it to the cell membrane and interacts there with the SRP receptor (FtsY). If the SRP system binds to the receptor, cleavage of the energy storage molecule GTP triggers further processes which ultimately anchor the protein in the membrane.
From cyanobacterium to chloroplast
In the cell fluid of bacteria, animals and plants, the SRP system consists of two components: the protein SRP54 and the ribonucleic acid SRP RNA. Several years ago, researchers found that the chloroplasts of higher plants, i.e. the photosynthetically active cell components, possess their own SRP system. It is very different from the system of the cell fluid because it has no SRP RNA. However, alongside SRP54 it also contains the protein SRP43, which occurs exclusively in chloroplasts. Scientists assume that chloroplasts originated from cyanobacteria, which initially lived in symbiosis with plant progenitor cells and were ultimately integrated into the plant cells. The scientists have now explored how the RNA-free SRP system of the chloroplasts originated from the RNA-containing SRP system of the bacteria.
Plant kingdom bioinformatically examined
With the aid of bioinformatics, the Bochum biologists and Dr. Magnus Rosenblad of Gothenburg University first examined which representatives in the plant kingdom have which components of the SRP system in their chloroplasts. "We were surprised that many organisms from unicellular green algae to mosses and ferns possess the gene for the SRP RNA in their chloroplasts", says Danja Schünemann. "The only exceptions are the higher plants, which have lost this gene". For them, the SRP system consists solely of the proteins SRP54 and SRP43. Interestingly though, SRP43 also occurs in the chloroplasts of lower plants, which are still equipped with SRP RNA.
SRP RNA in moss has partially lost its function
In collaboration with several groups of the Collaborative Research Centre SFB 642 at the RUB, Dr. Chantal Träger investigated the biochemistry of the moss Physcomitrella patens, which is among the lower plants. Physcomitrella has all the conceivable components of the SRP system in its chloroplasts: both the evolutionarily old components SRP54 and SRP RNA, as well as the more recent evolutionary protein SRP43. However, the SRP RNA of the moss chloroplasts forms a longer loop than the bacterial SRP RNA. This altered structure apparently prevents it from regulating the cleavage of GTP. Physcomitrella patens thus contains the evolutionarily old SRP RNA, which has largely lost certain functions. The SRP system of the chloroplasts of Physcomitrella patens therefore represents the transition between bacteria and higher plants. An X-ray structure analysis also revealed that the SRP receptor (FtsY) of the moss already has properties of the protein of higher plants.
C. Träger, M.A. Rosenblad, D. Ziehe, C. Garcia-Petit, L. Schrader, K. Kock, C.V. Richter, B. Klinkert , F. Narberhaus, C. Herrmann, E. Hofmann, H. Aronsson, D. Schünemann (2012): Evolution from the prokaryotic to the higher plant chloroplast Signal Recognition Particle: the Signal Recognition Particle RNA is conserved in plastids of a wide range of photosynthetic organisms, The Plant Cell, DOI: 10.1105/tpc.112.102996
Ruhr-University Bochum: http://www.ruhr-uni-bochum.de
This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.
New evidence shows that catastrophic climate change probably destroyed 96% of species at the end of the Permian period. It could happen again
Up to 120,000 animals are feared dead in central Kazakhstan, and still the cause is not known. Vets are investigating three main possibilities
New study predicts glaciers in the Everest region could decline by 70 to 99 percent over the next eight decades
The Obama administration announces a regulation it says is aimed at protecting the country's rivers, lakes and other waterways from pollution. But critics say it's a massive regulatory overreach.
The Antarctic ozone hole would have been 40% bigger and a hole over the Arctic would have opened up if ozone-depleting chemicals had not been phased out, according to research.
Storms and other extreme weather in Mexico, Texas, Oklahoma and Alaska could be the first signs that climate's bad boy is back with a vengeance
China has rapidly cleaned up its coal plants. Now comes the hard part.When William Latta first came to China, in 2005, he intended to look for companies to acquire for the French power giant Alstom. He wound up creating his own.
Scientists artificially inseminated a 100-year-old Yangtze giant softshell turtle, the last known female of her species
Researchers have identified a third gene that causes congenital insensitivity to pain when mutated
Ragweed pollen is the bane of many lives in the US, and climate change could help the plant become much more common in Europe by 2050