H2SO4Hurts - Brian Krueger, PhD H2SO4Hurts - Brian Krueger, PhD http://www.labspaces.net/view_blog.php?ID=15 Mon, 18 Mar 2024 23:37:33 -0500 Labspaces.net RSS Blog Feed http://www.labspaces.net/view_blogs.php http://www.labspaces.net/images/badge_large.png H2SO4Hurts - When Whole Genome Sequencing Doesn’t Give Us the Whole Genome <table> <tr><td> Image courtesy of Shutterstock If you’re an avid follower of popular science in today’s news media, you might have noticed a recurring theme.&amp;nbsp; Genomics is everywhere.&amp;nbsp; On an almost weekly basis, the New York Times, the New Yorker, Forbes and a myriad of other outlets are publishing stories with overly optimistic ledes about doctors and gene sequencers being replaced by apps ; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1627/When_Whole_Genome_Sequencing_Doesn___t_Give_Us_the_Whole_Genome Sun, 01 Mar 2015 13:00:54 -0600 H2SO4Hurts - 5 predictions for #AGBT15 <table> <tr><td> The Advances in Genome Biotechnology conference starts tomorrow in Marco Island, FL.&amp;nbsp; Twitter and the blogs have been a flurry of speculation about what the major vendors will present at this years’ meeting.&amp;nbsp; In previous years we’ve seen the introduction of new, “disruptive” technologies such as the ion torrent platform, the Oxford Nanopore Minion and the PacBio RS.&amp;n; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1626/__predictions_for__AGBT__ Tue, 24 Feb 2015 19:23:19 -0600 H2SO4Hurts - Have we entered the era of the 1 year sequencer release cycle? *Updated* <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/54b56380669ee1421173632_thumb.jpg" width="80" height="53.866666666667" border="0" /> </td><td> Yesterday marked the kickoff of the JP Morgan Healthcare Conference in San Francisco.&amp;nbsp; Following last years’ lead, Illumina once again used this platform as an opportunity to announce the release of a number of new products including 4 “New” sequencing systems ahead of the more scientifically focused Advances in Genome Biotechnology conference in February.&amp;nbsp; At this same tim; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1625/Have_we_entered_the_era_of_the___year_sequencer_release_cycle___Updated_ Tue, 13 Jan 2015 12:42:59 -0600 H2SO4Hurts - Illumina's $1000 Genome* <table> <tr><td> Illumina, the world leader in short read DNA sequencing, made a series of very big announcements yesterday at the JPMorgan Healthcare Conference.&amp;nbsp; These developments have many sequencing labs around the world excited and worried all at the same time.&amp;nbsp; The excitement comes from the fact that it appears on the surface that Illumina has broken the $1000 genome* barrier – the worry; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1623/Illumina_s_______Genome_ Wed, 15 Jan 2014 11:34:15 -0600 H2SO4Hurts - Ch-ch-ch-changes... <table> <tr><td> It's been a long run and we have a strong readership of the press releases but sadly I no longer have the time or the interest to continue posting press releases on the site.  My additional work commitments here at Duke have really limited the amount of time I can devote to this and grabbing the press releases every night/morning for an hour or two just became tedious.  I'd like to spend my free; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Misc http://www.labspaces.net/blog/1620/Ch_ch_ch_changes___ Wed, 29 May 2013 18:34:14 -0500 Featured - A coming of age for PacBio and long read sequencing? #AGBT13 <table> <tr><td> Aside from the dubstep pumping out of the Roche and Agilent booths, the volume of AGBT has been somewhat muted.&amp;nbsp; There was no grand offering of new hardware or over the top promises of sequencing genomes on what now appear to be vaporware USB thumb drives.&amp;nbsp; This is my first in person experience of AGBT, so as a virgin it seems for the most part to be rooted in the science despite; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1618/A_coming_of_age_for_PacBio_and_long_read_sequencing___AGBT___ Sat, 23 Feb 2013 13:10:01 -0600 Featured - Next Generation Sequencing rapidly moves from the bench to the bedside #AGBT13 <table> <tr><td> As the second day of AGBT kicked off, it became quite clear that this meeting would be dominated by medical genomics.&amp;nbsp; There were a few talks sprinkled in about gut or sewer microbiomes but the vast majority of the talks the last two days have been on clinical genomic sequencing.&amp;nbsp; This is fine by me since it’s exactly what we do in the Genomic Analysis Facility in Duke’s Cent; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1617/Next_Generation_Sequencing_rapidly_moves_from_the_bench_to_the_bedside__AGBT__ Fri, 22 Feb 2013 15:02:07 -0600 Featured - #AGBT day one talks and observations: WES/WGS, kissing snails, Poo bacteria sequencing <table> <tr><td> Today I dusted off my luggage and headed down to the annual Advances in Genome Biology and Technology meeting in Marco Island, FL.  Historically, this meeting has been the Detroit Auto Show of Genomics where companies and labs release their beautiful shiny new products and methodologies.  In past years, attendees were showered with fireworks displays and epic swag bags.  The tone this year is p; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1616/_AGBT_day_one_talks_and_observations__WES_WGS__kissing_snails__Poo_bacteria_sequencing Wed, 20 Feb 2013 23:57:41 -0600 Featured - Got fetal DNA on the brain? <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/5065cde804f3c1348849128_thumb.jpg" width="80" height="68.8" border="0" /> </td><td> Human fetus in amniotic sac One of the big stories that blew up on the internet the other day was the publication of some results that reportedly show that females who have birthed males have male DNA in their brain.&amp;nbsp; That’s pretty cool stuff!&amp;nbsp; This isn’t uncommon, it’s called microchimerism, or the deposition of cells or DNA from the fetus to the mother or vice versa.&amp; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1601/Got_fetal_DNA_on_the_brain_ Fri, 28 Sep 2012 11:33:14 -0500 Featured - Memes about 'junk DNA' miss the mark on paradigm shifting science <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/504a2f028ac931347038978_thumb.jpg" width="80" height="48" border="0" /> </td><td> Credit: Shutterstock In 1990, the scientific community embarked on a landmark experiment to completely sequence the human genome.&amp;nbsp; At the time, it was assumed that knowing the exact sequence of the human genome would provide scientists all of the information they ever wanted to know about genomics and how DNA contributes to human disease.&amp;nbsp; At least this is how the project was pr; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Genetics http://www.labspaces.net/blog/1599/Memes_about__junk_DNA__miss_the_mark_on_paradigm_shifting_science Fri, 07 Sep 2012 12:36:31 -0500 Featured - So, you've dropped a cryovial or lost a sample box in your liquid nitrogen container...now what? <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/502d724b353ae1345155659_thumb.jpg" width="80" height="106.66666666667" border="0" /> </td><td> Is it a hoe? Is it a shovel? It&#039;s bound to happen in every lab. &amp;nbsp;Someone is going to get distracted and for whatever reason a box full of tubes or tubes themselves are going to accidentally get dropped in the lab&#039;s liquid nitrogen container. &amp;nbsp;A lot of people might say, "Screw it," and leave those samples on the bottom of the tank. &amp;nbsp;This might be a good solutio; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Technology http://www.labspaces.net/blog/1596/So__you_ve_dropped_a_cryovial_or_lost_a_sample_box_in_your_liquid_nitrogen_container___now_what_ Thu, 16 Aug 2012 17:32:00 -0500 Featured - Did the Internet Save Science from Arsenic Poisoning? <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4f2aacccbaf5a1328196812_thumb.jpg" width="80" height="49.6" border="0" /> </td><td> GFAJ-1 Credit: Wolfe-Simon et al Almost a year and a half ago, NASA ignited a media firestorm after it announced the discovery of a new organism with alien implications.&amp;nbsp; The whole fiasco began when a scientist found a new bacteria in Mono Lake that could grow in the presence of high concentrations of toxic compounds.&amp;nbsp; These types of bacteria are not uncommon on earth.&amp;nbsp;; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Science Politics http://www.labspaces.net/blog/1589/Did_the_Internet_Save_Science_from_Arsenic_Poisoning_ Wed, 11 Jul 2012 12:31:23 -0500 Featured - A peril of "Open" science: Premature reporting on the death of #ArsenicLife <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4f2aacccbaf5a1328196812_thumb.jpg" width="80" height="49.6" border="0" /> </td><td> GFAJ-1 Bacteria Open science is a wonderful concept, but what happens when reporters start writing stories on data that has not been properly reviewed and vetted by the scientific establishment?&amp;nbsp; Before this week, I had never really considered this question.&amp;nbsp; Open science at its core is a wonderful utopian idea where scientists do their work in the open and publish their noteboo; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Science Politics http://www.labspaces.net/blog/1555/A_peril_of__quot_Open_quot__science__Premature_reporting_on_the_death_of__ArsenicLife Thu, 02 Feb 2012 09:42:54 -0600 H2SO4Hurts - YouTube: Just a (PhD) Dream <table> <tr><td> &lt;iframe width="560" height="315" src="http://www.youtube.com/embed/RK43NtL7b-k" frameborder="0" allowfullscreen&gt;&lt;/iframe&gt;; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Agriculture http://www.labspaces.net/blog/1514/YouTube__Just_a__PhD__Dream_ Thu, 27 Oct 2011 13:06:11 -0500 H2SO4Hurts - Antineoplastons? You gotta be kidding me! <table> <tr><td> Watch where you step, you might be knee-deep in some bullshit son! So I came across a movie about Stanislaw Burzynski and his controversial antineoplastons treatment. So I&#039;m pretty sure you are scratching your head wondering what an antineoplaston is? Apparently Burzynski created this convoluted phrase to use instead of simply saying, its a peptide. But take it from top here gang. In 1968, B; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Agriculture http://www.labspaces.net/blog/1512/Antineoplastons___You_gotta_be_kidding_me_ Thu, 27 Oct 2011 13:06:11 -0500 H2SO4Hurts - Slides - From the Bench to the Blogosphere: Why every lab should be writing a science blog <table> <tr><td> Here are the slides from the presentation I gave on Monday.&amp;nbsp; We recorded a video, but I&#039;m not sure how it turned out.&amp;nbsp; I have a feeling the audio is going to be bad so I might just sit down and do it over again this weekend on my laptop. Two of the slides are movies.&amp;nbsp; The first is a clip from "Flock of DoDos" where some lady says scientists are horrible communica; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Agriculture http://www.labspaces.net/blog/1508/Slides___From_the_Bench_to_the_Blogosphere__Why_every_lab_should_be_writing_a_science_blog Wed, 19 Oct 2011 10:03:32 -0500 Featured - Fact Checking AARP: Why soundbytes about shrimp on treadmills and pickle technology are misleading <table> <tr><td> AARP put out a commercial a few months ago deriding wasteful spending in Washington.&amp;nbsp; Unfortunately, the soundbytes don&#039;t accurately represent the full story behind the spending.&amp;nbsp; Have a watch before continuing. The main points of this mis-infomercial are: We&#039;re wasting money on a Brazillian corn institute, Poetry in Zoos, Shrimp on treadmills, and pickle technolog; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Science Politics http://www.labspaces.net/blog/1507/Fact_Checking_AARP__Why_soundbytes_about_shrimp_on_treadmills_and_pickle_technology_are_misleading Mon, 17 Oct 2011 09:00:00 -0500 Featured - MHV68: Mouse herpes, not mouth herpes, but just as important <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4e9b5ada43d2a1318804186_thumb.jpg" width="80" height="62.933333333333" border="0" /> </td><td> In the current political climate it has become clear that science is a major target of Republican directed budget cuts. However, the soundbytes of politics do not represent the importance of science in our lives.&amp;nbsp; Because of this, I think it&#039;s extremely important that we explain why some of our model systems are so important for understanding how viruses and ultimately human diseas; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Microbiology http://www.labspaces.net/blog/1506/MHV____Mouse_herpes__not_mouth_herpes__but_just_as_important Mon, 17 Oct 2011 08:33:38 -0500 Featured - @DonorsChoose update: Pictures of the materials we bought being used!! <table> <tr><td> Wow!&amp;nbsp; Mrs. Irish has posted pictures of her students using the microscope, slides and workbooks that we helped purchase for her classroom.&amp;nbsp; This is exactly why we work so hard to try to bring in donations through the DonorsChoose program. There are still 60 or so unfunded projects on our giving page, sp please stop by and help in any way you can. ; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Science Politics http://www.labspaces.net/blog/1503/_DonorsChoose_update__Pictures_of_the_materials_we_bought_being_used__ Fri, 14 Oct 2011 09:37:13 -0500 H2SO4Hurts - @DonorsChoose project: Launch a Rocket of Success <table> <tr><td> Mr. Bradham in class Today&#039;s featured DonorsChoose project is: Launch a Rocket of Success.&amp;nbsp; Mr. Bradham is looking to buy rocket kits for his science class to teach his students about physics and space exploration.&amp;nbsp; He says that his students are, "eager for knowledge. Unfortunately, they lack the adequate financial means to provide for supplies that could further their unde; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Space http://www.labspaces.net/blog/1492/_DonorsChoose_project__Launch_a_Rocket_of_Success Wed, 05 Oct 2011 09:15:40 -0500 H2SO4Hurts - Is this supposed to be a feature, @NPGnews ? <table> <tr><td> I know, know, paper is so 1990 but I just wanted to pull a paper over at Nature to read during seminar later.&amp;nbsp; Unfortunately I used the big fat "Print" button on the manuscript page.&amp;nbsp; This is what I got: Notice anything funny about the figures? I guess next time I&#039;ll just download the PDF and print it that way.&amp;nbsp; However, even I know that if you stick blocks of tex; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Internet http://www.labspaces.net/blog/1491/Is_this_supposed_to_be_a_feature___NPGnews__ Tue, 04 Oct 2011 14:26:39 -0500 H2SO4Hurts - A dose of batshit crazy: Bachmann would drill in the everglades if elected president <table> <tr><td> &lt;iframe frameborder="0" width="480" height="276" src="http://www.dailymotion.com/embed/video/xkrmrd"&gt;&lt;/iframe&gt;&lt;br /&gt;&lt;a href="http://www.dailymotion.com/video/xkrmrd_bachmann-says-she-d-consider-everglades-drilling_news" target="_blank"&gt;Bachmann Says She&amp;#039;d Consider Everglades Drilling&lt;/a&gt; &lt;i&gt;by &lt;a href="http://www.dailymotion.com/associatedpress" targ; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Environment http://www.labspaces.net/blog/1462/A_dose_of_batshit_crazy__Bachmann_would_drill_in_the_everglades_if_elected_president Mon, 29 Aug 2011 15:15:58 -0500 H2SO4Hurts - A true day in lab <table> <tr><td> Here&#039;s a true timelapse video of a day in lab.&amp;nbsp; Pictures were taken every minute for 24 hrs.&amp;nbsp; The video goes from about 4am to 4am the next day. And because someone asked...The images were taken with a GoProHD Hero camera and then compiled in Windows Live MovieMaker.&amp;nbsp; Images are displayed for 0.1 seconds.; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Research http://www.labspaces.net/blog/1443/A_true_day_in_lab Wed, 10 Aug 2011 07:57:17 -0500 H2SO4Hurts - A day in the lab... <table> <tr><td> &lt;iframe width="560" height="349" src="http://www.youtube.com/embed/FeSlLBzq53Y" frameborder="0" allowfullscreen&gt;&lt;/iframe&gt; Just messing around with timelapse on my GoPro.&amp;nbsp; This is only about 2 hours before the battery died.&amp;nbsp; Maybe tomorrow I&#039;ll set it up for 24hr and plug it into a nearby computer for power.; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Agriculture http://www.labspaces.net/blog/1440/A_day_in_the_lab___ Mon, 08 Aug 2011 15:00:12 -0500 H2SO4Hurts - More Troubleshooting <table> <tr><td> A week or so ago someone forgot to close the door on our enzyme freezer tightly.&amp;nbsp; I had just ordered $1,000 worth of NEB enzymes to make high throughput sequencing libraries too...&amp;nbsp; Before the meltdown, I made a couple of test libraries to be sure that the protocol was worked out. One of my test libraries with a perfect library smear.&amp;nbsp; We extract the DNA in between the ; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Molecular Biology http://www.labspaces.net/blog/1396/More_Troubleshooting Wed, 13 Jul 2011 09:52:58 -0500 H2SO4Hurts - End to the sonication saga <table> <tr><td> A quick update on the side project since I&#039;m procrastinating writing a short fellowship grant...&amp;nbsp; About a month ago I realized why my sonications were not working.&amp;nbsp; It turns out that when I moved to Florida from my Graduate work at Iowa, I didn&#039;t update my ChIP protocol to reflect a reagent change.&amp;nbsp; At Iowa, we had been using 16% paraformaldehyde as our crossli; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Research http://www.labspaces.net/blog/1357/End_to_the_sonication_saga Tue, 07 Jun 2011 14:56:12 -0500 H2SO4Hurts - Reflecting on a childhood whack-a-loon <table> <tr><td> In a recent episode of the new TV show, Happy Endings (Episode on HuLu), one of the characters, Dave, gets super excited when he runs into and reconnects with his favorite high school teacher.&amp;nbsp; The only problem is that the teacher turns out to be an alcoholic douche, but Dave spends the entire episode fawning over the guy until he realizes that the teacher is just an underachieving l; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Careers http://www.labspaces.net/blog/1343/Reflecting_on_a_childhood_whack_a_loon Fri, 20 May 2011 12:27:42 -0500 H2SO4Hurts - University of Iowa holds Science Writing Symposium <table> <tr><td> &amp;nbsp; The University of Iowa will be holding s science writing symposium tomorrow (April 27th).&amp;nbsp; The symposium will be webcast LIVE at this address from 1PM to 5PM CST: https://webapps1.healthcare.uiowa.edu/webcast/Default.aspx There&#039;s a login screen now, but that will disappear once the talks start.; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Internet http://www.labspaces.net/blog/1314/University_of_Iowa_holds_Science_Writing_Symposium Tue, 26 Apr 2011 09:10:34 -0500 H2SO4Hurts - Sonication success?? <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4dac90333a3b01303154739_thumb.jpg" width="80" height="74.666666666667" border="0" /> </td><td> I&#039;ve been working on this project on and off for a few months now.&amp;nbsp; You may remember my previous post about attempting sonication trials.&amp;nbsp; Unfortunately, things in lab have been busy trying to get a new graduate student started and troubleshooting problems with other experiments so I haven&#039;t had as much time to devote to this project as I would like.&amp;nbsp; I&#039;ve; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Research http://www.labspaces.net/blog/1302/Sonication_success__ Mon, 18 Apr 2011 14:35:26 -0500 H2SO4Hurts - Circle of life <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4d821787203811300371335_thumb.jpg" width="80" height="60.266666666667" border="0" /> </td><td> Today I noticed my Tomato Clownfish acting a little friskier than usual.&amp;nbsp; A few days ago I saw a white bump under the female&#039;s anal fin and thought it might be a fungal infection because it had a goofy gray tinge to it.&amp;nbsp; I figured I&#039;d just wait and see what developed.&amp;nbsp; Well today I noticed that it was much bigger and longer...It was her ovipositor (egg laying t; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Marine Biology http://www.labspaces.net/blog/1257/Circle_of_life Thu, 17 Mar 2011 09:55:27 -0500 H2SO4Hurts - Curing a plague: Cryptocaryon irritans <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4d77b073ab1071299689587_thumb.jpg" width="80" height="92.8" border="0" /> </td><td> Every once in a while I find myself having a "grass is greener" moment in science.&amp;nbsp; I sit at the computer thinking about all of the annoying things in lab that aren&#039;t working and wonder, "If I could answer any question, given unlimited resources, what would I choose to study?"&amp;nbsp; This, my friends, is where I prove my unhealthy obsession with fish.Credit: Colorni and Burgess, 1; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Marine Biology http://www.labspaces.net/blog/1243/Curing_a_plague__Cryptocaryon_irritans Wed, 09 Mar 2011 11:13:02 -0600 H2SO4Hurts - Video: First new fish in 6 months!! <table> <tr><td> Things have started to calm down a bit on my weekends so I&#039;ve had more time to attend to fish.&amp;nbsp; One of the crappiest things about owning a saltwater fish tank is that you can&#039;t just add fish to your system immediately.&amp;nbsp; The fish come from the ocean covered in parasites, so you have to take extra special care of new additions to be sure they&#039;re clear of parasites an; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Marine Biology http://www.labspaces.net/blog/1222/Video__First_new_fish_in___months__ Wed, 02 Mar 2011 09:37:41 -0600 Featured - Don't ever get a sun tan...on your eye balls <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4d482cc43a6be1296575684_thumb.jpg" width="80" height="60.266666666667" border="0" /> </td><td> "This is the one thing you never want to mess around with.&amp;nbsp; You&#039;ll wake up in the middle of the night crying like a baby and you&#039;ll have no idea why."&amp;nbsp; These were the sage words of my undergraduate advisor, it&#039;s too bad they got discarded with everything else that I learned in undergrad.------My scientific career has been filled with plenty of misadventures and scr; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Research http://www.labspaces.net/blog/1162/Don_t_ever_get_a_sun_tan___on_your_eye_balls Tue, 01 Feb 2011 10:14:54 -0600 H2SO4Hurts - #Scio11: Collaborative tools in industry and academia <table> <tr><td> I&#039;m finally coming out of my Scio11 coma.&amp;nbsp; It was a super exciting weekend filled with talks about technology, blogging, and new media.&amp;nbsp; On Saturday, Kristy Meyer, Dr. Isis and I led a panel discussion on the use of new communication tools in academic and industry science.The discussion covered a wide range of topics.&amp;nbsp; One of the first that was brought up was how ne; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Technology http://www.labspaces.net/blog/1117/_Scio____Collaborative_tools_in_industry_and_academia Wed, 19 Jan 2011 11:22:56 -0600 H2SO4Hurts - The first step is the most important <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4d1ced6e7ada41293741422_thumb.jpg" width="80" height="60.266666666667" border="0" /> </td><td> I&#039;m beginning a new project in lab.&amp;nbsp; It&#039;s a series of ChIP-seq experiments and the first step to doing ChIP-seq properly is optimizing sonication conditions.&amp;nbsp; Here&#039;s a trial run with the sonicator I plan on using.&amp;nbsp; The DNA shown in the gel is from cells containing latent herpes virus.&amp;nbsp; We&#039;re looking to shear the DNA so that the bulk of it is ; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Molecular Biology http://www.labspaces.net/blog/1075/The_first_step_is_the_most_important Thu, 30 Dec 2010 14:37:53 -0600 Featured - Have we really found a stem cell cure for HIV? <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4d08f4d29e8e91292432594_thumb.jpg" width="80" height="69.333333333333" border="0" /> </td><td> Yesterday there was some buzz over at Huffington Post about a stem cell cure for HIV.&amp;nbsp; I first ran across the article via a link a friend of mine had posted on Facebook.&amp;nbsp;&amp;nbsp; The HuffPo piece is scant on details, so I’ll provide a quick run down on what’s going on here. But first, a lesson in HIV virology… HIV Virion Credit: NIH Human immunodeficiency virus (HIV) wa; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Molecular Biology http://www.labspaces.net/blog/1053/Have_we_really_found_a_stem_cell_cure_for_HIV_ Wed, 15 Dec 2010 11:40:24 -0600 Featured - This paper saved my graduate career <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4d078b7323b891292340083_thumb.jpg" width="80" height="51.2" border="0" /> </td><td> In a previous post I mentioned that I spent the first year of graduate school working on a dead end project that was going nowhere.&amp;nbsp; I’m going to present and discuss the findings of the paper that changed the direction of my thesis project and ultimately lead to the completion of my PhD.In the fall of 2006, I was finishing up the last few experiments on a project trying to link P53 (a v; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Molecular Biology http://www.labspaces.net/blog/1048/This_paper_saved_my_graduate_career Tue, 14 Dec 2010 09:43:41 -0600 Featured - Valium or Sex: How do you like your science promotion <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4cebe8fc0dd601290529020_thumb.jpg" width="80" height="51.2" border="0" /> </td><td> With the launch of this year’s “Rock Stars of Science” campaign, there’s been a lot of talk about how to best promote science.&amp;nbsp; I’m no marketing guru, but I am a scientist.&amp;nbsp; This latest campaign is better than last years&#039;, only because it’s more diverse, but I think it really misses the boat.&amp;nbsp; Is the public really going to be inspired by a couple of pict; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Science Politics http://www.labspaces.net/blog/956/Valium_or_Sex__How_do_you_like_your_science_promotion Tue, 23 Nov 2010 10:19:30 -0600 H2SO4Hurts - A wedding pic. <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4ce34d4d207c21289964877_thumb.jpg" width="80" height="112.53333333333" border="0" /> </td><td> &amp;nbsp;&amp;nbsp;I&#039;m back! And here&#039;s an early treat from my photographer and good friend, Todd Adamson; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Misc http://www.labspaces.net/blog/927/A_wedding_pic_ Tue, 16 Nov 2010 12:00:00 -0600 H2SO4Hurts - To rule by terror <table> <tr><td> When I started graduate school at Iowa, I went in there with a chip on my shoulder.  They didn’t choose me, I chose them.  They weren’t a highly ranked “elite” institution, so to make my mark I had to work for the biggest and the best that Iowa had to offer, or so I thought.  I sought out the highest profile researchers at Iowa and picked the one that best aligned with my interests.  N; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Careers http://www.labspaces.net/blog/903/To_rule_by_terror Tue, 09 Nov 2010 14:32:05 -0600 Featured - Summary Feed: What I would be doing if I wasn't doing science <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4cab2a17f26a41286285847_thumb.jpg" width="80" height="101.33333333333" border="0" /> </td><td> Today is: "What I&#039;d be doing if I wasn&#039;t doing science" blog post theme day.&amp;nbsp; The goal of this post theme is to let our readers get to know who we are and what our non-scientific interests are. &amp;nbsp; Credit: Gaeten Lee/CC3.0 1. DamnGoodTechnician says that she&#039;d probably have majored in sociology and become an administrative assistant if it wasn&#039;t for her high ; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Careers http://www.labspaces.net/blog/663/Summary_Feed__What_I_would_be_doing_if_I_wasn_t_doing_science Wed, 06 Oct 2010 09:00:00 -0500 H2SO4Hurts - "You have more Hobbies than anyone I know" <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4cab7fe20b9dc1286307810_thumb.jpg" width="80" height="62.933333333333" border="0" /> </td><td> I thought about this question probably everyday of my graduate school career.&amp;nbsp; My days usually went like this: 1. Get to lab at 7am2. Start 12hr experiment3. 7pm, experiment failed4. 7:15pm set it all up again for tomorrow Eventually I got everything to work but that 12hr period in the middle was filled with: "I bet me engineer friends don&#039;t have to deal with this shit, and they&#; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Careers http://www.labspaces.net/blog/671/_quot_You_have_more_Hobbies_than_anyone_I_know_quot_ Tue, 05 Oct 2010 14:46:56 -0500 H2SO4Hurts - Hiccupping Hubris <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4c9a1dbfe413d1285168575_thumb.jpg" width="80" height="0" border="0" /> </td><td> Credit: Jens Lagner I&#039;m going to preface this by saying that I am not a medical expert. I don&#039;t even begin to pretend I know anything about medicine or how to cure diseases. I do watch those cheesy "Untold stories of the ER" shows on Discovery and TLC though, and sometimes I partially remember things they say about diagnoses. This story begins back when I was finishing up my PhD at Iow; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Health http://www.labspaces.net/blog/574/Hiccupping_Hubris Wed, 22 Sep 2010 00:00:00 -0500 H2SO4Hurts - A death in the family :( <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4c97b7a972eb9_thumb.jpg" width="80" height="68.8" border="0" /> </td><td> I came into lab yesterday to a disaster. I took a look at my quarantine tank and it was filled with a white bacterial bloom, the bottom of the tank was covered in what looked like leftover food and fish poop, and the water smelled like rotting fish. To top it off, the Powder Blue Tang could not right itself, and was swimming upside down and in circles. For those of you that have never owned fis; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Marine Biology http://www.labspaces.net/blog/554/A_death_in_the_family___ Mon, 20 Sep 2010 12:00:00 -0500 H2SO4Hurts - The new lab fish! <table> <tr><td> This is kind of stupid, but I thought I&#039;d share a video of my new lab fish. Who knows if they&#039;ll survive quarantine, but we can bask in their beauty for a while. The blue-ish one is a powder blue tang approximately 3" long and the yellow one is one of my favorite fish of all time! It&#039;s a yellow long nose butterfly fish. The tang is covered in a pretty common parasite so they hav; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: YouTube http://www.labspaces.net/blog/518/The_new_lab_fish_ Fri, 10 Sep 2010 14:25:56 -0500 H2SO4Hurts - What I wish I knew...Before applying to graduate school <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/thumbs/4c868f1ccfaa5_thumb.jpg" width="80" height="71.466666666667" border="0" /> </td><td> Credit: Rohan BaumannI’m totally late to this party. I spent the morning writing my rebuttal to DrugMonkey and Co, doing the news, and cranking out a few pesky experiments. Ah, to live the life, right? Anyway, I’ve noticed that all of the good topics are now taken so I have to scrub the bottom of the bucket. I think one of the most important decisions I made in my scientific career was whe; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Careers http://www.labspaces.net/blog/511/What_I_wish_I_knew___Before_applying_to_graduate_school Tue, 07 Sep 2010 14:32:53 -0500 H2SO4Hurts - Stopping viruses by targeting human proteins <table> <tr><td> <img src="http://www.labspaces.net/pictures/blog/12777395924c28c248d01e0_blog.jpg" width="80" height="0" border="0" /> </td><td> One of the biggest problems facing the eradication of hard to kill viruses such as HIV is that viruses mutate readily. A standard technique for creating lasting immunity against viruses is the creation of vaccines. These have been used for years to eradicate a multitude of viruses. There are three standard types of vaccines that have been used in the past. There are attenuated viral vaccines w; (read more) </td> </tr> </table> <br>Source: Brian Krueger, PhD - Discipline: Molecular Biology http://www.labspaces.net/blog/153/Stopping_viruses_by_targeting_human_proteins Tue, 29 Jun 2010 00:00:00 -0500