Posted by: Genomic Repairman
Posted on: Thu, Oct 14, 2010, 10:57 am CDT
Have and its doing about the same thing. Also construct has RFP so, I know that its in there too.
Nikki as a proof of concept that your shRNA is working you could make or obtain a cell line that overexpresses a tag version of your protein, do your RNAi and measure KD to see how effective your shRNA is?