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Price Lab
Lab Information
Location:
University of Iowa
Department:
Biochemistry
Address:3120 MERFIowa City, IA 52246 USAKeywords:
p-tefb, hexim, transcription, ttf2
Homepage: http://www.u...u/~pricelab/
Lab Members (3) See all?
Protocols
Stocking Cells
Tue, Mar 25, 2008, 6:21 pm CDT
This is a cell stocking protocol that I have used on HeLa and HCT116 cells
Keywords: stocking cells, mammalian, HeLa
Western Blot
Sat, Aug 26, 2006, 2:33 pm CDT
A general western blot protocol from gel transfer to antibody probing
Keywords: Western blot, probe, antibodies, transfer
E.coli transformation
Mon, Mar 24, 2008, 2:34 pm CDT
General protocol for transforming bacteria. This one is tailored for DH5alpha
Keywords: transformation, e. coli
Oligo Radiolabeling
Tue, Mar 25, 2008, 1:50 pm CDT
Radioactively label oligonucleotides for EMSA or Northern blot analysis
Keywords: Radioactivity, oligonucleotides
DNA Ethanol Precipitation
Tue, Mar 25, 2008, 1:52 pm CDT
2 methods for precipitating DNA after isolation. Ammonium acetate and sodium acetate methods.
Keywords: Ethanol, precipitation, DNA, nucleic acid isolation
Glycerol Gradient analysis of protein complexes
Tue, Mar 25, 2008, 1:54 pm CDT
This is the Price Lab's glycerol gradient protocol. I don't think we've published a paper that doesn't use this technique.
Keywords: Glycerol Gradient, protein complex
Hybridization of Northern Blot Probes
Tue, Mar 25, 2008, 1:56 pm CDT
This is an "old school" northern blot hybridization protocol. I now use the very expensive UltraHybe Reagent from ambion
Keywords: Northern blot, RNA, hybridization
Hybridization of Northern Blot Probes - UltraHybe Protocol
Tue, Mar 25, 2008, 1:57 pm CDT
This is my UltraHybe protocol for hybridizing Northern Blot probes to their targets
Keywords: Northern blot, RNA, hybridization
Immunoprecipitation using protein G sepharose
Tue, Mar 25, 2008, 2:01 pm CDT
General protocol for protein G sepharose immunoprecipitation of protein complexes. Could be adapted for other media such as protein A.
Keywords: Immunoprecipitation
Immunoprecipitation using Dynal Beads
Tue, Mar 25, 2008, 2:02 pm CDT
This is a Dynal bead immunoprecipitation protocol. Much less messy than using other IP media and with significantly less bead loss than other methods.
Keywords: Dynal, immunoprecipitation
In vitro transcription using T7 RNA polymerase
Tue, Mar 25, 2008, 2:03 pm CDT
This is a general protocol for an in vitro transcription reaction using T7 RNA polymerase.
Keywords: In vitro transcription, T7 RNA polymerase
Northern Blot Semi-Dry Transfer
Tue, Mar 25, 2008, 2:05 pm CDT
General protocol for a northern blot transfer using a semi-dry apparatus. Refer to the Price Lab buffer protocol for buffer recipes.
Keywords: Northern blot, RNA, transfer, semi dry
DNA Gel solutions
Tue, Mar 25, 2008, 2:10 pm CDT
TAE, DNA loading buffer, TE
Keywords: TAE, DNA loading buffer, TE
Other Solutions
Tue, Mar 25, 2008, 2:11 pm CDT
HGE, HKGE, PBS, tRNA Sarkosyl, Water Saturated Phenol
Keywords: HGE, HKGE, PBS, tRNA Sarkosyl, Water Saturated Phenol
RNA Gel Solutions
Tue, Mar 25, 2008, 2:12 pm CDT
TBE, Urea, RNA loading buffer
Keywords: TBE, Urea, RNA loading buffer
Protein Gel Solutions
Tue, Mar 25, 2008, 2:13 pm CDT
Tris Glycine Running Buffer, Loading Buffer, Western buffers, APS
Keywords: Tris Glycine Running Buffer, Loading Buffer, Western buffers, APS
Price Lab Stock Solutions
Tue, Mar 25, 2008, 2:15 pm CDT
HEPES, EDTA, KCl, NaCl, MgCl2, DTT, SDS, Tris pH 7.5, Tris pH 8, Tris pH 10.4
Keywords: HEPES, EDTA, KCl, NaCl, MgCl2, DTT, SDS, Tris pH 7.5, Tris pH 8, Tris pH 10.4
Protein Gels
Tue, Mar 25, 2008, 2:16 pm CDT
Protein gel protocol for SDS-PAGE, gel recipes for 6, 9, 12, and 15% acrylamide. See Price Lab stock solutions for all buffers
Keywords: Protein Gel, SDS, PAGE
Denaturing Urea RNA gel
Tue, Mar 25, 2008, 2:18 pm CDT
Recipe for a denaturing Urea 6% acrylamide RNA gel
Keywords: RNA, gel, denaturing
Fast Silver Stain Protocol
Tue, Mar 25, 2008, 2:19 pm CDT
This is the Price Lab's fast silver stain protocol. Approx 1hr.
Keywords: Silver stain, protein
RNA Extraction
Tue, Mar 25, 2008, 2:20 pm CDT
This is an extraction protocol using invitrogen's Trizol reagent
Keywords: RNA isolation extraction
Purifification of HIS-tagged protein
Tue, Mar 25, 2008, 2:26 pm CDT
This is the Price Lab protocol for the purification of HIS-tagged proteins
Keywords: Protein purification, HIS tag
T7 Expression of HIS tagged proteins
Tue, Mar 25, 2008, 2:27 pm CDT
This is a protocol for expressing HIS-Tagged proteins in E. coli
Keywords: Protein Expression, E. coli
Publications
Nguyen D Krueger BJ Sedore SC Brogie JE Rogers JT Rajendra TK Saunders A Matera AG Lis JT Uguen P Price DH. 2012. The Drosophila 7SK snRNP and the essential role of dHEXIM in development... 0:
Krueger BJ Varzavand K Cooper JJ Price DH. 2010. The mechanism of release of P-TEFb and HEXIM1 from the 7SK snRNP by viral and cellular activators includes a conformational change in 7SK..PLoS ONE. 5:
Krueger BJ Jeronimo C Roy BB Bouchard A Barrandon C Byers SA Searcey CE Cooper JJ Bensaude O Cohen EA Coulombe B Price DH. 2008. LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated..Nucleic Acids Res.. 36:2219-29
Sedore SC Byers SA Biglione S Price JP Maury WJ Price DH. 2007. Manipulation of P-TEFb control machinery by HIV: recruitment of P-TEFb from the large form by Tat and binding of HEXIM1 to TAR..Nucleic Acids Res.. 35:4347-58
Michels AA Fraldi A Li Q Adamson TE Bonnet F Nguyen VT Sedore SC Price JP Price DH Lania L Bensaude O. 2004. Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor..EMBO J.. 23:2608-19
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