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Cyanine5 NHS ester, tech questions, citations - Newsletter

Traz Ardagau
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Cyanine5 NHS ester, tech questions, citations - Newsletter
Thu, Oct 23, 2014, 2:01 pm CDT


Lumiprobe Newsletter - Cyanine5 NHS ester, tech questions, citations lumiprobe.comWhen was the last time you visited Lumiprobe's updated website?
  Take a look ! Lumiprobe manufactures and sells over 80 reagents offered in 1mg, 5mg, 25 mg, 50 mg and 100 mg sizes plus bulk and larger sizes available! Each page has pricing, availablility, information on structure, absorption and emission spectra, downloadable MSDS, and general properties.

Want a quick guide to what binds with what? amine reactive, azides, thiol mono, NHS esters, Azdes, maleimides, ROX, carboxylic, water soluble, BDF FL or Pyrene and more!
Lumiprobe's catalog by alphabetic listing

Custom Synthesis Want something not in the catalog? Need a large volume? Lumiprobe has created dyes since 2006. Often a research lab requests a custom probe, that is added to the Lumiprobe catalog. What do you need to use in your research? Talk with Lumiprobe tech support and something can be created for you, at no additional development charges.

Downloadable Lumiprobe Catalog and reference book:

Lumiprobe supports all size research labs!

Quality Control

Lumiprobe manufactures and sells its own reagents. Quality control has improved over the years and never sacrifices quality. All chemical materials produced, including intermediates and of course final products, undergo analysis. Lumiprobe quality control uses a combination of two gold-standard analytical methods for the assay of purity and identity our chemicals: NMR, and LC-MS. For our users, knowing that the compound works for the desired application is often the main thing. We regularly check that the reagents work as expected - for example, that they label proteins, stain gels, and provide fluorescent signals. Each batch manufactured by Lumiprobe is labeled with batch number and a sample stored. Lumiprobe can assay it again if something goes wrong at customer's lab. Lumiprobe maintains an electronic quality system which keeps records of all materials and intermediates produced and analyzed at our labs. All manufacturing steps are well documented, too. This allows to obtain consistent results and maintain outstanding quality over time. Certificate of Analysis (CoA) with analytical data is available for every batch manufactured.



Featured Reagent : Delve into Dye NHS esters (Reactive dyes) ROX NHS ester, pure 6- isomer | BDP FL NHS ester | Sulfo-Cyanine3 NHS ester
Sulfo-Cyanine 5 NHS ester | Sulfo-Cyanine7 NHS ester| Cyanine3 NHS ester
Cyanine3.5 NHS ester | Cyanine5 NHS ester | Cyanine5.5 NHS ester | Cyanine7 NHS ester | Cyanine7.5 NHS ester cyanine5 NHS ester Limelight on Cyanine5 NHS ester During the last few years, Cyanine5 flurophore (analog of Cy5) has become an incredibly popular label in life science research and diagnostics. Fluorophore emission has maximum in red region, where many CCD detectors have maximum sensitivity, and biological objects have low background. Dye color is very intense, therefore quantity as small as 1 nanomol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5 NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require co-solvent, and has very similar fluorescent properties.


Citations of interest - Take a look!
Would you like your paper featured on Lumiprobe citation page? 

Use of Lumiprobe Cyanine dyes for diagnostic tool and drug development: 1) Liu, Z.; Miller, S.J.; Joshi, B.P.; Wang, T.D.
In vivo targeting of colonic dysplasia on fluorescence endoscopy with near-infrared octapeptide.
Gut, 013, 62, 395-403. doi:10.1136/gutjnl-2011-301913
Peptides were labeled with peptides were labelled with Cyanine5.5, a NIR dye, and characterised by mass spectra. Research demonstrated the created near-infrared (NIR) peptide is highly specific for colonic adenomas on fluorescence endoscopy in vivo and sub-cellular resolution images were achieved
Full paper:

2)Xie, M.; Shi, H.; Ma, K.; Li, B.; Shen, S. Wang, X.; Jin, Y
Hybrid nanoparticles for drug delivery and bioimaging: Mesoporous silica nanoparticles functionalized with carboxyl groups and a near-infrared fluorescent dye.
J. Colloid and Interface Sci., 2013, 395, 306-314. doi: 10.1016/j.jcis.2013.01.001 nanoparticles in drug delivery and live cell imaging

The development of a drug delivery system with fluorescent biolabels is important in anti-cancer drug delivery application due to the potential for simultaneous diagnosis and treatment of diseases. Here, we reported the synthesis and multiple functionalization of mesoporous silica nanoparticle (MSN) for bioimaging and controlled drug release. After the functionalization with carboxyl group, the nanoparticles exhibited much better dispersity and stability in aqueous solution than MSN. Furthermore, a substantial doxorubicin (DOX) loading level was achieved and DOX loaded nanoparticles exhibited noticeable pH-sensitive behavior with accelerated release of DOX in acidic environment. Compared with native DOX MSN, DOX MSN/COOH Cy5 exhibited enhanced intracellular uptake efficacy and stronger effect on killing tumor cells. Meanwhile, it was observed that the MSN/COOH Cy5 was able to locate in the cytoplasm of MCF 7 cells and could accumulate in tumor tissues for a long period of time. Overall, the functional nanoparticle could potentially be used for simultaneous controlled drug release and near-infrared fluorescent bioimaging

Tech Support: Lumiprobe offers FREE tech support before or after you order.
A few questions:
Could you compare sulfonated vs non-sulonated cyanine dyes? Read the Lumiprobe paper comparing sulfonated and nonsulfonated cyanine dyes   I'm looking at using Cyanine5 as a background dye in some PCR experiments to check for loading amounts of liquids into these experiments (add and scan on first cycle). Thus I'm looking for a non-reactive version of the dye to add in that will not interfere with proteins (such as polymerases) or DNA. Can Cy5-azide that is used for click chemistry be used for this application. Are there other possible Cyanine5 types that would be possible for this application.
Sulfo-Cyanine5 azide ( should be suitable for your application. While regular Cyanine5 azide has low water solubility, sulfo-Cyanine5 is very soluble in water. It does not seem to interact with normal biomolecules, and it has same fluorescence as other Cyanine5-containing probes.

Could you send me a quotation and delivery time for 25mg of 43020 Cyanine 5 NHS ester and its protocol?
Lumiprobe thanks you for your enquiry and sends formal quotes whenever requested. Each reagent on the Lumiprobe website
has its own webpage with information. Pricing, availability and information about Cyanine5 NHS ester is at:
Under General properties there is downloadable MSDS. The Cyanine5 NHS ester has chemical structure, absorption and emissions spectra. Protocol for NHS ester labeling of amino biomolecules and other protocols are at:    What Cy5 polimethyne dyes can be used in hematology analizer with flow citometry, to differential of leukocites in 5 parts? The light source used in the system is a semiconductor laser emitting red light at 633 nm
Yes, it is possible to use this cyanine dye with a flow cytometer. If your instrument has Cyanine5 channel then it is compatible. Cyanine5 and sulfo-Cyanine5 are suitable dyes for your instrument - you can use them with your cytometer/sorter.  Do you ever have a sulfhydryl reactive version of a sulfonated Cy3 or Cy5? Lumiprobe offers two sulfhydryl reactive water soluble cyanine dyes. sulfo cyanine3 maleimide and sulfo cyanine5 maleimide

Send your tech questions   Any comments on the newsletter? What information would you like in the next issue?


Cyanine5 NHS ester, tech questions, citations - Newsletter