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Storage of samples prior to RNA isolation

Thomas Joseph
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Storage of samples prior to RNA isolation
Mon, Sep 06, 2010, 9:29 pm CDT
Working with RNA is always a tricky thing. It's probably the thing I am most anal retentive about when it comes to the lab. Our lab not only has entire areas set aside for RNA work, we also have reserved pipetors (with tips) and reagents for the practice as well. Halting RNA degradation during an experiment is something we've also spent a lot of time considering. Since bacteria are pretty adept at responding rapidly to their environment, simply sticking a sample in a freezer won't do the trick. As those cells cool down, they will go into cold shock and the transcription profiles will start to change. RNA synthesis needs to halt immediately, as does RNA degradation (those molecules typically have a half-life of less than 120 seconds). This acetone/ethanol protocol does the trick in our hands. It's as quick and as cheap a solution as we've been able to come to. Not everyone can afford Ambion's RNALater by the gallon.

http://www.labspaces.net/protocols/616741283537722_protocol.pdf

Genomic Repairman
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Storage of samples prior to RNA isolation
Mon, Sep 06, 2010, 9:29 pm CDT
Nice tip, I'm definitely taking this one to heart since I do a bunch of RNA isolation.
Storage of samples prior to RNA isolation
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