Posted by: thecancergeek
Posted on: Sun, Oct 03, 2010, 3:19 am CDT
My lab does tons and tons of western blots, and I gotta give a shout out to Cell Signaling for their primary antibodies. Very few of them actually suck, and the ones that do, nobody else makes good ones either. We study the PI3K/mTOR pathway (among other things) so if you need an any advice on good ones let me know. Their secondaries are very good too, but pity they don't make many species, just anti-rabbit and anti-mouse. One Christmas Cell Signaling ran an awesome promotion something like $25 iTunes gift card for $200 of product, or something like that....the whole lab got $50 gift cards that year, since we stocked up on things we knew we would use.
Recently I've been trying some Epitomics monoclonals, and the 2 i've found have been hits: TSC2 and phospho-ATM (however this only works for human, as the datasheet indicates).
We also buy gels from BioRad, and don't have any complaints. In general they seem pretty reliable, and our rep will give us free ones if we occasionally have problems.
Dharmacon is my fave source of siRNA - the SMARTpools are what I normally get. Usually I see no increase in efficiency beyond 10nM making them last a pretty long time.