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Brian Krueger, PhD


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Friends (46): See all?

Eric Weatherford
University of Iowa

srinubabu gedela
OMICS Publishing Group

StephSK3
University of Iowa
Labs
Primary Lab

Renne Core Facility
University of Florida
Molecular Genetics and Microbiology

Price Lab
University of Iowa
Biochemistry
User Information
Positon:
Scientist
Homepage:
http://www.labspaces.net
Address:Insitute for Genomic MedicineNew York, NY 10032 USAKeywords:
next generation sequencing, genomics, transcription, gene expression
Projects:
Currently the Director of Genomic Analysis and Technical Operations for the Institute of Genomic Medicine at Columbia University Medical Center.
Interests:
High throughput sequencing (Illumina), Genetics, Genomics, Biochemistry, Molecular Biology, Cloning, Protein expression and purification, HTML, CSS, Javascript, PHP, MySQL, Search engine optimization
Heroes:
Rosalind Franklin.
Awards:
Daphne Schwartz award for excellence in biology (Bradley)

University of Iowa Molecular and Cellular Biology Training Grant (2006-2008)
Non-Scientific Interests:
Biking, hiking, weight lifting, Salt water fish tanks, Reef Tanks
Education History
University of Iowa
Iowa City, IA USA
6/2005 - 12/2009
Degree:
Ph.D. Molecular and Cellular Biology
Description of Work:
Work with P-TEFb and elucidate the function of LARP7
Bradley University
Peoria, IL USA
8/2001 - 6/2005
Degree:
B.S. Biology
Description of Work:
Worked mostly with plants and some animals.
Work History
Columbia University Medical Center
New York, NY USA
12/2014 - Present
Position:
Director of Genomic Analysis and Technical Operations
Description of Work:
I oversee the core facilities and drive technical advancement at the Institute for Genomic Medicine
Duke University
Durham, NC USA
11/2012 - 12/2014
Position:
Associate Director
Description of Work:
I run the Genomic Analysis Facility at Duke's Center for Human Genome Variation
University of Florida
Gainesville, FL USA
1/2010 - 11/2012
Position:
Lead Scientist
Description of Work:
I run a core lab that creates recombinant herpesviruses for the greater herpesvirus community.
USDA NCAUR
Peoria, IL USA
6/2003 - 6/2004
Position:
Research Assistant
Description of Work:
Played with yeast all day.
Publications
Nguyen D Krueger BJ Sedore SC Brogie JE Rogers JT Rajendra TK Saunders A Matera AG Lis JT Uguen P Price DH. 2012. The Drosophila 7SK snRNP and the essential role of dHEXIM in development... 0:
Krueger BJ Varzavand K Cooper JJ Price DH. 2010. The mechanism of release of P-TEFb and HEXIM1 from the 7SK snRNP by viral and cellular activators includes a conformational change in 7SK..PLoS ONE. 5:
Krueger BJ Jeronimo C Roy BB Bouchard A Barrandon C Byers SA Searcey CE Cooper JJ Bensaude O Cohen EA Coulombe B Price DH. 2008. LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated..Nucleic Acids Res.. 36:2219-29
Protocols
Stocking Cells
Tue, Mar 25, 2008, 6:21 pm CDT
This is a cell stocking protocol that I have used on HeLa and HCT116 cells
Keywords: stocking cells, mammalian, HeLa
Western Blot
Sat, Aug 26, 2006, 2:33 pm CDT
A general western blot protocol from gel transfer to antibody probing
Keywords: Western blot, probe, antibodies, transfer
E.coli transformation
Mon, Mar 24, 2008, 2:34 pm CDT
General protocol for transforming bacteria. This one is tailored for DH5alpha
Keywords: transformation, e. coli
Oligo Radiolabeling
Tue, Mar 25, 2008, 1:50 pm CDT
Radioactively label oligonucleotides for EMSA or Northern blot analysis
Keywords: Radioactivity, oligonucleotides
DNA Ethanol Precipitation
Tue, Mar 25, 2008, 1:52 pm CDT
2 methods for precipitating DNA after isolation. Ammonium acetate and sodium acetate methods.
Keywords: Ethanol, precipitation, DNA, nucleic acid isolation
Glycerol Gradient analysis of protein complexes
Tue, Mar 25, 2008, 1:54 pm CDT
This is the Price Lab's glycerol gradient protocol. I don't think we've published a paper that doesn't use this technique.
Keywords: Glycerol Gradient, protein complex
Hybridization of Northern Blot Probes
Tue, Mar 25, 2008, 1:56 pm CDT
This is an "old school" northern blot hybridization protocol. I now use the very expensive UltraHybe Reagent from ambion
Keywords: Northern blot, RNA, hybridization
Hybridization of Northern Blot Probes - UltraHybe Protocol
Tue, Mar 25, 2008, 1:57 pm CDT
This is my UltraHybe protocol for hybridizing Northern Blot probes to their targets
Keywords: Northern blot, RNA, hybridization
Immunoprecipitation using protein G sepharose
Tue, Mar 25, 2008, 2:01 pm CDT
General protocol for protein G sepharose immunoprecipitation of protein complexes. Could be adapted for other media such as protein A.
Keywords: Immunoprecipitation
Immunoprecipitation using Dynal Beads
Tue, Mar 25, 2008, 2:02 pm CDT
This is a Dynal bead immunoprecipitation protocol. Much less messy than using other IP media and with significantly less bead loss than other methods.
Keywords: Dynal, immunoprecipitation
In vitro transcription using T7 RNA polymerase
Tue, Mar 25, 2008, 2:03 pm CDT
This is a general protocol for an in vitro transcription reaction using T7 RNA polymerase.
Keywords: In vitro transcription, T7 RNA polymerase
Northern Blot Semi-Dry Transfer
Tue, Mar 25, 2008, 2:05 pm CDT
General protocol for a northern blot transfer using a semi-dry apparatus. Refer to the Price Lab buffer protocol for buffer recipes.
Keywords: Northern blot, RNA, transfer, semi dry
DNA Gel solutions
Tue, Mar 25, 2008, 2:10 pm CDT
TAE, DNA loading buffer, TE
Keywords: TAE, DNA loading buffer, TE
Other Solutions
Tue, Mar 25, 2008, 2:11 pm CDT
HGE, HKGE, PBS, tRNA Sarkosyl, Water Saturated Phenol
Keywords: HGE, HKGE, PBS, tRNA Sarkosyl, Water Saturated Phenol
RNA Gel Solutions
Tue, Mar 25, 2008, 2:12 pm CDT
TBE, Urea, RNA loading buffer
Keywords: TBE, Urea, RNA loading buffer
Protein Gel Solutions
Tue, Mar 25, 2008, 2:13 pm CDT
Tris Glycine Running Buffer, Loading Buffer, Western buffers, APS
Keywords: Tris Glycine Running Buffer, Loading Buffer, Western buffers, APS
Price Lab Stock Solutions
Tue, Mar 25, 2008, 2:15 pm CDT
HEPES, EDTA, KCl, NaCl, MgCl2, DTT, SDS, Tris pH 7.5, Tris pH 8, Tris pH 10.4
Keywords: HEPES, EDTA, KCl, NaCl, MgCl2, DTT, SDS, Tris pH 7.5, Tris pH 8, Tris pH 10.4
Protein Gels
Tue, Mar 25, 2008, 2:16 pm CDT
Protein gel protocol for SDS-PAGE, gel recipes for 6, 9, 12, and 15% acrylamide. See Price Lab stock solutions for all buffers
Keywords: Protein Gel, SDS, PAGE
Denaturing Urea RNA gel
Tue, Mar 25, 2008, 2:18 pm CDT
Recipe for a denaturing Urea 6% acrylamide RNA gel
Keywords: RNA, gel, denaturing
Fast Silver Stain Protocol
Tue, Mar 25, 2008, 2:19 pm CDT
This is the Price Lab's fast silver stain protocol. Approx 1hr.
Keywords: Silver stain, protein
RNA Extraction
Tue, Mar 25, 2008, 2:20 pm CDT
This is an extraction protocol using invitrogen's Trizol reagent
Keywords: RNA isolation extraction
Purifification of HIS-tagged protein
Tue, Mar 25, 2008, 2:26 pm CDT
This is the Price Lab protocol for the purification of HIS-tagged proteins
Keywords: Protein purification, HIS tag
T7 Expression of HIS tagged proteins
Tue, Mar 25, 2008, 2:27 pm CDT
This is a protocol for expressing HIS-Tagged proteins in E. coli
Keywords: Protein Expression, E. coli
Friends