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Thu, Oct 07, 2010, 7:58 am CDT
I have been trying for days to find the the best ( and cheapest unfortunately ) way to determine cytochrome P450 activity ( so not PCR or Western blot ) .
and I have been stumped so many websites with pay for scholarly articles that you just have no idea if they are any use at all ,
I don't even know if this is the right place to post it , but if anyone knows anything about it I would love if you would share that knowledge with me :-)
Thanks allot guys
/Roy
Sun, Oct 03, 2010, 5:43 pm CDT
We always used a luminometer assay from promega, but i don't think it was cheap. Maybe someone else has more experience with alternative techniques.
Thu, Oct 07, 2010, 6:09 am CDT
Thanks for the answer :-)
Thu, Oct 07, 2010, 7:58 am CDT
Kind of depends on the P450, since it's such a large family with wide range of substrates. If you're simply trying to determine if proper folding/heme incorporation, you might start with a simple CO binding spectrum.
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