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Press Release
Engineered bacteria effectively target tumors, enabling tumor imaging potential in mice
Thursday, January 26, 2012


(a) 2D bioluminescence co-registration. Athymic mouse bearing s.c. FLuc expressing FaDu tumour 7 days post IV administration of B. breve. Tumour FLuc = Green, Bacterial lux = orange. (b) 3D bioluminescence co-registration. 3D overlay of B. breve lux (orange) 10 days post administration to athymic mice bearing HCT116 FLuc (green) expressing tumours.

Tumor-targeted bioluminescent bacteria have been shown for the first time to provide accurate 3D images of tumors in mice, further advancing the potential for targeted cancer drug delivery, according to a study published in the Jan. 25 issue of the online journal PLoS ONE.

The specially engineered probiotic bacteria, like those found in many yoghurts, were intravenously injected into mice with tumors, after which the researchers took full body bioluminescent images. The 3D images revealed information about the number and location of the bacteria, to the level of precisely revealing where within the tumour the bacteria were living, providing much more information on the interaction of bacteria and tumors than was previously available using similar two-dimensional imaging methods.

According to the authors, led by Mark Tangney of University College Cork in Ireland, "before now, researchers used luminescence to provide an approximation of where a test organism was within the body, and would then follow up with multiple further experiments using different techniques to try to find a precise location". This new research suggests that such bacteria can be engineered to contain diagnostic or therapeutic agents that would be produced specifically within the tumor for targeted treatment.

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Cronin M, Akin AR, Collins SA, Meganck J, Kim J-B, et al. (2012) High Resolution In Vivo Bioluminescent Imaging for the Study of Bacterial Tumour Targeting. PLoS ONE 7(1): e30940. doi:10.1371/journal.pone.0030940

Public Library of Science: http://www.plos.org



Thanks to Public Library of Science for this article.

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Comments

Brian Krueger, PhD
University of Florida
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Like 0 Dislike
Thu, Jan 26, 2012, 2:22 pm CST

So glad they put the bacteria in there. I'm not sure I'd be able to find the tumor otherwise.  The detection threshold on this is pretty terrible.

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