I was trying to think back to my biggest lab mistake and although I have had quite a few minor mishaps (mis-loading lanes for a Western blot, not adding the right controls for a Q-RT-PCR) that lead to disastrous scientific outcomes (i.e. repeating weeks worth of sample acquisition), those mistakes aren't as entertaining as the one I have decided to feature.

Let me set the scene.  I was working as a technician straight out of my undergrad.  I had been with the lab for a few years and one of duties was autoclaving glassware, pipette tips etc.  Simple grunt work.

Or so you would think.

During my tenure with this lab we actually relocated our lab space to a brand new building and a few weeks after moving in they installed brand new autoclaves for our department.  Ooohh Ahhh.  They were state of the art, brand new and shiny (no gross brown stains, nothing oozing out of the seams, no strange odors at start up) and they came pre-programmed with standard dry and wet runs.  Pretty dummy proof.

Or so you would think.

I was actually excited to throw in my load of glassware and break in the virgin autoclave.  I loaded the beakers and flasks into the Nalgene pans we had always used for autoclaving, pressed 'Dry cycle' and away I went.  "Damn, we should hire an undergrad to do this.. any boob can do it."

Or so I thought.

I returned to the autoclave a few hours later and was greeted with the most god-awful smell.  I immediately knew something was wrong.  I quickly opened the (no longer brand new shiny) door and almost passed out.  There, lying directly on the autoclave rack was all my glassware, and underneath where the autoclave tray used to be?  Well, it looked like the aftermath of Ghostbusters v. The Stay Puft Marshmallow man:

The gigantic tub had completely melted and there was plastic hanging down from the rack, oozing down the walls and pooled on the bottom.  It had re-solidified and was like concrete. Some of the glassware had been fused to the rack and so there it was, in full glory for all to see, the name of my lab.

Now everyone was going to know which boob trashed the brand new autoclave.  I was so embarrassed.

In case you didn't realize it, Nalgene should only be autoclaved at 121 degrees. 99% of autoclaves use 121 degrees as their standard sterilization temperature, but it turns out that this autoclave was set at 134 degrees (often used for viruses and other highly resistant agents.)  No other autoclaves I had ever worked with used 134 as a pre-set, only as a programmable option.

I am sure I am not the only one who has ever made this mistake.  In fact there is evidence out there which proves that assumption correct, but I bet I am the first person to ever do it in a brand.spaking.new autoclave.

The department called Stericycle and they sent someone to remove the plastic, but the autoclave was out of commission for at least 2 weeks, and even the plastic was gone it was never the same.   The machine had lost it's shine.  The inside was permanently discolored and there was a funny chemical smell when you opened the door.

From that day forward I ALWAYS checked the cycle conditions on the autoclave.

This disaster occurred nearly 15 years ago and even though I do a lot less autoclaving now, it has forever changed the way I supervise autoclaving as lab manager.  I have switched over to using stainless steel autoclave baskets in order to ensure that this won't happen to anyone else (like the lowly undergrad summer help or new graduate student who may not be as vigilant about checking the autoclave settings.)

I try to use my mistake to make autoclaving so easy a caveman could do it.

Or so you would think.