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Post Archive
2019 (0)2010 (241)
December (20)

Have a little personality on Friday...
Friday, December 10, 2010

Thursday is going to be epic...
Thursday, December 9, 2010

Good Night, and Good Luck
Wednesday, December 8, 2010

Ask Genomic RepairMom...Child Birth
Wednesday, December 8, 2010

Wednesday is definitely a rollercoaster
Wednesday, December 8, 2010

12 Months of Blogging Meme
Tuesday, December 7, 2010

LabSpaces Fantasy Football Challenge: Week 13
Tuesday, December 7, 2010

Don't Run Around On A Tuesday
Tuesday, December 7, 2010

A rhetorical question and science fail...
Monday, December 6, 2010

Sue
Monday, December 6, 2010

It definitely doesn't feel like summer...
Monday, December 6, 2010

A Friday Recipe... Stuffed Jalapenos
Friday, December 3, 2010

A Friday morning haiku for you...
Friday, December 3, 2010

Friday better not be your time of dying...
Friday, December 3, 2010

Music Warz--Fuck all you emos--Punk is where its at...
Thursday, December 2, 2010

Is this a sign of things to come...
Thursday, December 2, 2010

Speaking to the next generation...
Thursday, December 2, 2010

Apparently we must have used hypocritical evangelical coders...
Thursday, December 2, 2010

Have an Unbelievable Thursday
Wednesday, December 1, 2010

Don't ge a Gigolo on Wednesday, its just not right.
Wednesday, December 1, 2010
November (44)

LabSpaces Fantasy Football Challenge: Week 12
Tuesday, November 30, 2010

It Might Get Loud On Tuesday
Tuesday, November 30, 2010

Do you know what the Spellcheck button is?
Tuesday, November 30, 2010

Update: I think I'm dying...Science in Motion...
Monday, November 29, 2010

Feel The Noise On a Monday
Monday, November 29, 2010

The jail story...
Wednesday, November 24, 2010

Don't be talking that shit on a Wednesday..
Wednesday, November 24, 2010

The dark danger and human cost of drug development
Tuesday, November 23, 2010

LabSpaces Fantasy Football Challenge: Week 11
Tuesday, November 23, 2010

Thrift Store T Shirt Tuesday
Tuesday, November 23, 2010

Request for Reprint...Should I put on my throwback jersey too?
Monday, November 22, 2010

Moving Boxes, we aren't going a damn place...
Monday, November 22, 2010

Does your brain feel like its going to Pop on a Monday?
Monday, November 22, 2010

Friday Wrap-Up and Links
Friday, November 19, 2010

Believe Me, Its Friday
Friday, November 19, 2010

An Open Letter To The Guy Who Tried To Mug Me...
Thursday, November 18, 2010

Who the hell said it was Thursday?
Thursday, November 18, 2010

Ask Genomic RepairMom...Retractions.
Wednesday, November 17, 2010

Are you in the house?
Wednesday, November 17, 2010

Pay me fuckers
Tuesday, November 16, 2010

LabSpaces Fantasy Football Challenge: Week 10
Tuesday, November 16, 2010

Don't Feed the Animals
Tuesday, November 16, 2010

A Tuesday Morning Book Review...
Tuesday, November 16, 2010

On which I make my return
Monday, November 15, 2010

One of the best discussions on Climate Change...Ever.
Monday, November 15, 2010

Guess who's back?
Monday, November 15, 2010

Lets keep on Rocking on a Friday...
Friday, November 12, 2010

Don't Rock the Boat on a Thursday
Thursday, November 11, 2010

Don't get too Hooked on Wednesday
Wednesday, November 10, 2010

Don't Wine on a Tuesday
Tuesday, November 9, 2010

Caribbean Monday
Monday, November 8, 2010

I'm out...
Monday, November 8, 2010

We call this a teachable moment...
Friday, November 5, 2010

Can't help that its Friday
Friday, November 5, 2010

A comparative study on pain medication reactions (n=2)
Thursday, November 4, 2010

Dear students applying for fellowships...
Thursday, November 4, 2010

Don't be Tempted on a Thursday...
Thursday, November 4, 2010

Ask Genomic RepairMom...Elections
Wednesday, November 3, 2010

Working it out from home on Wednesday...
Wednesday, November 3, 2010

LabSpaces Fantasy Football: Week 8
Tuesday, November 2, 2010

Hope you find Satisfaction on a Tuesday...
Tuesday, November 2, 2010

Tales of the Genomic Repairman: DNS
Monday, November 1, 2010

The fall of Agamemnon and the rise of Ajax: Is the PI being cheap?
Monday, November 1, 2010

Don't be Trippin on a Monday...unless you want to.
Monday, November 1, 2010
October (47)

Is the PI being cheap?
Friday, October 29, 2010

Friday Save
Friday, October 29, 2010

Nobel Prize Blunder...
Thursday, October 28, 2010

Don't be gray on a Thursday...
Thursday, October 28, 2010

Be a Wiz on a Wednesday
Wednesday, October 27, 2010

Ask Genomic RepairMom.... Vaccines
Tuesday, October 26, 2010

Things learned from my Society meeting...
Tuesday, October 26, 2010

LabSpaces Fantasy Football: Week 7
Tuesday, October 26, 2010

Attack my Site...I'm Gonna Knock You Out...
Tuesday, October 26, 2010

Write, write, write...
Monday, October 25, 2010

Hold on I'm Coming to play you some music on a Monday...
Monday, October 25, 2010

Get off this Friday...
Friday, October 22, 2010

Dear Graduate Student, let me list the reasons why I shall beat you with a stick...
Thursday, October 21, 2010

Slow down, you don't have to Run on a Thursday...
Thursday, October 21, 2010

Could you be loved on a Wednesday...
Wednesday, October 20, 2010

Ask Genomic RepairMom...Big Bang Theory...
Wednesday, October 20, 2010

It was only a matter of time before I got off my ass...
Tuesday, October 19, 2010

LabSpaces Fantasy Football: Week 6
Tuesday, October 19, 2010

Is Everything Zen on a Tuesday?
Tuesday, October 19, 2010

10 Commandments of Poster Presentations...
Tuesday, October 19, 2010

Shout it out on Monday
Monday, October 18, 2010

Pinch me its Friday...
Friday, October 15, 2010

Donor's Choose: Give me your lunchmoney!
Thursday, October 14, 2010

So I know I slagged off on a vendor but now its time to give one props...
Thursday, October 14, 2010

Did you see Fireflies on a Thursday?
Thursday, October 14, 2010

New Series: Ask Genomic RepairMom...Stem Cells
Wednesday, October 13, 2010

Make Wednesday A Magic Moment...
Wednesday, October 13, 2010

The Tribe of Science loses a Warrior
Tuesday, October 12, 2010

LabSpaces Fantasy Football: Week 5
Tuesday, October 12, 2010

Hey Man, quit Ramblin on a Tuesday
Tuesday, October 12, 2010

Cocaine is not a good way to start the week off...
Monday, October 11, 2010

Candidacy Exam: Last one out gets the lights...
Monday, October 11, 2010

Who would have ever thought that Gatorade was pro-evolution...
Sunday, October 10, 2010

Candidacy Exam: I think I'm qualified.
Friday, October 8, 2010

Giving you guys some culture...One Semester of Spanish, Spanish Love Song
Friday, October 8, 2010

I'm sorta Research Blogging...Insight into the function of PALB2
Thursday, October 7, 2010

Chances Are I'm bringing you old guy radio today
Thursday, October 7, 2010

You only knew it was a matter of time before I went off again...
Wednesday, October 6, 2010

Welcome to Wednesday
Wednesday, October 6, 2010

LabSpaces Fantasy Football: Week 4
Tuesday, October 5, 2010

What I'd be doing, if I wasn't doing this: A fishing story!
Tuesday, October 5, 2010

Keep Moving on Tuesday
Tuesday, October 5, 2010

A little Dave Holland recommendation for Tideliar
Monday, October 4, 2010

My mom reads my blog, Happy Birthday Genomic RepairMom
Monday, October 4, 2010

More than a feeling on Monday Morning: I rocked this song in Rock Band
Monday, October 4, 2010

Candidacy Exam: Fourth and inches...
Sunday, October 3, 2010

I'm running late because I got caught on the Radar Gun
Friday, October 1, 2010
September (45)

A man can only take so much bluegrass, time to rock!
Thursday, September 30, 2010

Don't be running down the street hungry as a hound on Wednesday...
Wednesday, September 29, 2010

How not to foster a scientific collaboration...
Tuesday, September 28, 2010

LabSpaces Fantasy Football: Week 3
Tuesday, September 28, 2010

In Spite of Tuesday
Tuesday, September 28, 2010

Candidacy Exam: Someone check his pulse
Monday, September 27, 2010

Stomp it out on a Monday
Monday, September 27, 2010

Update: To the coworker who saw me lose my shit tonight...
Friday, September 24, 2010

Hoping for No Rain on a Friday
Friday, September 24, 2010

Don't have a bad Thursday
Thursday, September 23, 2010

Don't be Greedy, enjoy your Wednesday
Wednesday, September 22, 2010

What is your opinion on reuse of a figure in a journal? Misconduct?
Tuesday, September 21, 2010

LabSpaces Fantasy Football Challenge: Week 2
Tuesday, September 21, 2010

Taking it back old school with The Tokens on a Tuesday
Tuesday, September 21, 2010

I've got a case of the Mondays...
Monday, September 20, 2010

A little Rebel Yell on Monday
Monday, September 20, 2010

An Open Letter to Bio-Rad...This is going to fuck up any chance of a sponsorship...
Sunday, September 19, 2010

A lawyer is calling stem cells outmoded?
Thursday, September 16, 2010

Happy Mexican Independence Day & Potential Darwin Award
Thursday, September 16, 2010

Is Science the Love of Your Life?
Thursday, September 16, 2010

Got it made in the shade on Wednesday?
Wednesday, September 15, 2010

Can you go the distance on Tuesday?
Tuesday, September 14, 2010

LabSpaces Fantasy Football Challenge: Week 1
Monday, September 13, 2010

Candidacy Exam: Damnit go away!
Monday, September 13, 2010

Don't break down on a Monday.
Monday, September 13, 2010

Good grief, its Friday!
Friday, September 10, 2010

Guest Post: The Easy Pub
Thursday, September 9, 2010

You don't know how it feels on a Thursday
Thursday, September 9, 2010

Funniest Comment Contest Part 2
Wednesday, September 8, 2010

Stay with me on a Wednesday
Wednesday, September 8, 2010

I wish I knew: A Tale of 2.5 stories...
Tuesday, September 7, 2010

Lets just be friends on a Tuesday...
Tuesday, September 7, 2010

In case you weren't signed up...
Monday, September 6, 2010

Come on ride that train on a Monday...
Monday, September 6, 2010

Candidacy Exam: Clocks ticking yo!
Monday, September 6, 2010

You want to guest post on LabSpaces?
Friday, September 3, 2010

Watch out for the small print on a Friday
Friday, September 3, 2010

Are you actually working with a neuronal line?
Friday, September 3, 2010

Groups
Thursday, September 2, 2010

Whats that smell and how should I mentor it?
Thursday, September 2, 2010

Travelling Riverside on a Thursday
Thursday, September 2, 2010

LFG...
Wednesday, September 1, 2010

Contest Winners!!!
Wednesday, September 1, 2010

Go Your Separate Ways on a Wednesday
Wednesday, September 1, 2010

My first (and best ever) conference...
Wednesday, September 1, 2010
August (75)

Mentoring a mentor?: Get it in writing.
Tuesday, August 31, 2010

Warning: Its Tuesday
Tuesday, August 31, 2010

I've survived a month of blogging.
Monday, August 30, 2010

New Yorker Article on Francis Collins and the Stem Cell Ruling
Monday, August 30, 2010

Crunch!
Monday, August 30, 2010

Can't Relate to a Monday
Monday, August 30, 2010

Female Science Bloggers...
Monday, August 30, 2010

Potential blogger drink err meet up?
Friday, August 27, 2010

Candidacy Exam: Scheduling, scheduling, scheduling
Friday, August 27, 2010

Friday morning special...
Friday, August 27, 2010

Dear IACUC inspector...
Thursday, August 26, 2010

The State of My Blog
Thursday, August 26, 2010

The Genomic Repairman's Book Club
Thursday, August 26, 2010

Wishing you were home on a Thursday
Thursday, August 26, 2010

Here is where you get to win the liquor
Wednesday, August 25, 2010

Epic Fail on a macroscale level.
Wednesday, August 25, 2010

Some recent big news in the DNA Repair field
Wednesday, August 25, 2010

Don't get caught asleep at the wheel on a Wednesday morning
Wednesday, August 25, 2010

Funniest comment, formerly most obnoxious contest...
Tuesday, August 24, 2010

Dear Trainee, the clock is ticking...
Tuesday, August 24, 2010

Cleanliness is next to...
Tuesday, August 24, 2010

Tuesday morning got you feeling numb
Tuesday, August 24, 2010

An Open Letter to Core Director
Monday, August 23, 2010

Conference Calls
Monday, August 23, 2010

Don't be blue on a Monday!
Monday, August 23, 2010

LabSpaces NFL Pick 'Em Challenge
Friday, August 20, 2010

Its Friday In Spite of Ourselves
Friday, August 20, 2010

The Genomic Repairman gets a pat on the back from the boss and a boot in the ass
Friday, August 20, 2010

Legit Science Question Here Folks
Thursday, August 19, 2010

Rise up on a Thursday Morning
Thursday, August 19, 2010

Dumping on technicians...
Thursday, August 19, 2010

Antibody Naming Contest Winners
Wednesday, August 18, 2010

Trying to find some music for tomorrow, like yesterday
Wednesday, August 18, 2010

ncRNA: Oh the splendor, oh the horror!
Wednesday, August 18, 2010

Still want in on Battle of the Science Blogs
Tuesday, August 17, 2010

Another twofer on a Tuesday morning.
Tuesday, August 17, 2010

Linked Out?
Tuesday, August 17, 2010

Data Embargos
Monday, August 16, 2010

I need a remedy for this sunburn
Monday, August 16, 2010

Cell culture question for the readers...
Monday, August 16, 2010

A letter to the other graduate student in my lab...
Friday, August 13, 2010

Friday morning groovin by the Fratellis
Friday, August 13, 2010

The Apprentice: The Finale!
Friday, August 13, 2010

Candidacy Exam: The Runaround
Thursday, August 12, 2010

Shake off them shoes if you got the Thursday morning blues...
Thursday, August 12, 2010

Update to the aged RO1
Wednesday, August 11, 2010

Candidacy Exam: The Series
Wednesday, August 11, 2010

Shameless product plug
Wednesday, August 11, 2010

Quality is when you...
Wednesday, August 11, 2010

Honestly I didn't think I'd make it to Wednesday
Wednesday, August 11, 2010

A LabSpaces Prayer
Tuesday, August 10, 2010

A two for Tuesday with the boys of Cracker and Camper Van Beethoven
Tuesday, August 10, 2010

You can hold an RO1 for 27 fucking years!
Tuesday, August 10, 2010

Name my antibody!
Monday, August 9, 2010

Antineoplastons? You gotta be kidding me!
Monday, August 9, 2010

Monday morning beats by Morcheeba
Monday, August 9, 2010

So I'm at work today so I figure I owe you a song...
Sunday, August 8, 2010

Well since I'm working on a Saturday
Saturday, August 7, 2010

So I got tagged in a damn meme
Friday, August 6, 2010

What are you doing to promote science to the public?
Friday, August 6, 2010

A chilled tune for Friday and an extra track
Friday, August 6, 2010

Say goodbye to film.
Friday, August 6, 2010

Long Lost Paper
Thursday, August 5, 2010

Feel alive with Live on a Thursday morning.
Thursday, August 5, 2010

A Forum For Discussion
Wednesday, August 4, 2010

Some Wonderful Music for a Wednesday Morning
Wednesday, August 4, 2010

Is this really elevating your science?
Wednesday, August 4, 2010

Helen Larson my ass!
Wednesday, August 4, 2010

2010 LabSpaces NFL Challenge & Banner Contest
Wednesday, August 4, 2010

Balance...You gotta be kidding me.
Tuesday, August 3, 2010

Feeling Stuck on a Tuesday?
Tuesday, August 3, 2010

A Note On Peer Review
Monday, August 2, 2010

Monday Morning Soothing Tunes and Moving Music
Monday, August 2, 2010

Candidacy Exam Blues
Monday, August 2, 2010

How do you balance the job and life?
Monday, August 2, 2010
July (10)
Blogger Profile

Genomic Repairman

The Genomic Repairman is currently a Ph.D. student who escaped from the deep south, and studies DNA damage and repair through biochemical and genetic approaches. He intends to use pine away about his scientific interests and rant about the things (and there are lots of them) that annoy him.

My posts are presented as opinion and commentary and do not represent the views of LabSpaces Productions, LLC, my employer, or my educational institution.

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Awesome Stuff
Views: 215 | Comments: 14
Last by Genomic Repairman on Oct 08, 2010, 10:33am
Maybe Namnezia and I will be the only ones who get into this but enjoy "Chances Are" by the soothing Johnny Mathis who was not only an accomplished singer but also a decathlete.



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Views: 589 | Comments: 14
Last by biochem belle on Oct 08, 2010, 11:30am
So this week has been a relative sea of calm for me as I've been working on my presentation for my oral defense for my qualifying exam on Friday. That was until yesterday. Since we had a deluge of posts yesterday I was saving this one up for Wednesday and was too lazy to get around to writing it up until now. Thank goodness I have two hands, that way I use them to choke two people at the same time. In the left hand would be my boss (he has a skinnier neck and its my weaker hand) and in the right would be our other graduate student (thicker neck to match the skull of mountain goat therefore necessitating the strong hand).

Lets begin with my boss, you have been nothing short of a pain in the neck to me during this exam process. You have piled work high on me, not really given me the required dispensation to work on my exam and also had me training technicians while going through this hellacious process. Its nice that you are now taking interest in my work, but fucking with centrifuge while I'm using it is not cool. I just want to remind you that there are two very precious samples spinning at 90,000 fucking rpm and you playing with the rotor settings (that can be applied) while the rotor is spinning because you like to tinker as you say. You want me to st . . . More
Views: 168 | Comments: 2
Last by Nikkilina on Oct 06, 2010, 9:10am


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Views: 210 | Comments: 11
Last by biochem belle on Oct 06, 2010, 10:43am
I think someone's on a streak. For the second week in a row Dr. Becca comes out with a win.



Too Much Bourbon FTW, Again!

And here go the rest of us:

2. DGT (showing strong)

3. Odyssey (making moves)

4. PLS (WTF man, how many weeks are you going to rent the 4th place in this challenge?)

5. Dr. O (Your Boyz were resting this week, but you weren't)

6. LabSpaces badboy Tideliar (climbing out of mediocrity, watch out next week)

7. Lady Scientist (Nice bounce back from the no show last week).

8 . . . More
Views: 308 | Comments: 14
Last by JanedeLartigue on Oct 07, 2010, 12:45pm
Not to start this post off in a macabre fashion but I'd like to recite the following poem to begin:

Crossing the Bar

by Alfred Tennyson

Sunset and evening star, And one clear call for me! And may there be no moaning of the bar, When I put out to sea. But such a tide as moving seems asleep, Too full for sound and foam, When that which drew from out the boundless deep Turns again home! Twilight and evening bell, And after that the dark! And may there be no sadness of farewell, When I embark; For though from out our bourn of Time and Place The flood may bear me far, I hope to see my Pilot face to face When I have crost the bar. This poem, written in 1889, has been read aloud at the funeral of all of my family members for over 100 years. This poem was originally chosen by my great great grandfather shortly before his own demise to be read at his funeral service. This poem had special meaning to him and to the rest of us in my family because we have all been at some point in our lives sea faring people. Many of relatives were dock workers, merchant marines, or ship captains. My own father was a sailor in a foreign navy while my own existence up until the past two years has existed within a stones . . . More
Views: 118 | Comments: 0
Enjoy Keep Moving from the experimental rock band Trocadero out of Cambridge, MA.



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Views: 204 | Comments: 6
Last by Genomic Repairman on Oct 06, 2010, 10:25pm
Tideliar was grooving to Miles Davis hugely influential album Bitches Brew the other day. That album is pretty interesting because Davis brought the other musicians into the studio without really giving them notice and they mostly played this chaotic free-form of jazz music. This was also one of the first albums to ever "loop" part of a track for instance the bass line. Also if you can get ahold of the old vinyl, this album has some of the most beautiful artwork (they went for an Afro-centric motherland theme that is just incredible) that I have seen.

But enough about Miles Davis, you came to here about the legendary Dave Holland, a British jazz bandleader, whose music has spanned across five decades. He plays in quintet, he plays sextet, hell he'll play with a large group of people. Enjoy this bit of music history.






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Views: 541 | Comments: 14
Last by Brian Krueger, PhD on Oct 04, 2010, 7:09pm
So I mentioned to my sister that I was blogging and she ended up showing my mom the blog.  To put it kindly, my mother was initially mortified and embarrassed by the things that I wrote.  This goes all the way back to my childhood where I was polite in public but really had no respect for adults.  Even going so far as to tell my parents that they were responsible for me and thus my bad behavior was really just a poor reflection of them.  I'm sure this attitude will bite me in the ass when I have my own family (crossing fingers not for a while).  So lets rundown my mother's feelings about LabSpaces and I'd like to preface that my mother's knowledge of science ends at salt tastes salty.  While she deplores my liberal use of profanity, she finds Tideliar's charming (must be the Brit fucker's charming use of foul language).  She thinks Brian works too hard and needs more of tan (you are looking a little WASPy in that profile photo buddy).  She recommends that Disgruntled Julie tells her boss to kiss her ass and takes a vacation.  And she thinks Geeka should get her MS.

My mom thinks the iPad contest is cool and would try to compete for it but for two small problems.  She isn't techy savy at all (hunt and pecker at the keyboard who has to get my sister to download a . . . More
Views: 224 | Comments: 8
Last by Genomic Repairman on Oct 04, 2010, 6:21pm
For never playing before, I racked up a 90% on medium. Not too shabby.



Funny version from Scrubs



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Views: 406 | Comments: 18
Last by Nikkilina on Oct 05, 2010, 8:14am
So here is my update on this shenanigans:

I turned in my proposal on Friday mere minutes before the deadline and sent off a copy for Turnitin analysis.  I don't care about the analysis but I do not like Turnitin based on the fact that my work is intellectual property and they are ripping me off.  Papers submitted to them are placed into their catalog and thus used for plagiarism analysis.  They derive financial benefit for using my paper and they charge the institution for use of their bullshit software which doesn't even really tell you this much.  A lot of what pops up as plagiarism for us as we have seen in the past is materials and methods and most of that is self-plagiarism.  I mean how many different ways can you say that you run a western blot when you are trying to write concise sentences in order to make the page limits?

But I digress.  The paper is in and this Friday is my oral presentation, so that is what I am spending most of my time on.  I hope by Tuesday to have a rough presentation put together in order to run through with some folks.  But the gist of it is you give a short background, show a few slides of preliminary data, and really kick the show off by describing your hypothesis, and finally launch into your aims.  This is sort of a g . . . More
Views: 217 | Comments: 10
Last by Nikkilina on Oct 04, 2010, 3:12pm
"Radar Gun" by The Bottle Rockets



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Views: 157 | Comments: 0
Shoot to Thrill by AC/DC.  Nuff said.



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Views: 126 | Comments: 0
A daily listen on my playlist is this "Honey Babe" from blues/folk artist and one of the best guitar players that I have ever seen, Brooks Williams.  Enjoy.



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Views: 1013 | Comments: 16
Last by Genomic Repairman on Oct 13, 2010, 6:50pm
So we've had a lab from really huge famous university that has sort of been pestering us about collaborating on a project.  Their angle is they want to use a mouse model that we have developed in the lab and need our technical assistance as they are neophytes when it comes to our field and what we do.  To generate some data they sent out a senior research scientist (SRS) from their lab to do some preliminary work.  What did it require from us?  A ton of mice and a lot of initial prep work from two of our technicians to get this off the ground.  So end result the SRS was here for two days and used our technicians, mice, and supplies to do their work.  Today before leaving the SRS gave us a brief seminar to our lab and others on what exactly they are doing and why they need us.  

The seminar was pretty interesting, the lab's work is quite interesting and novel.  At one point during the seminar she is talking about previously presenting at a conference where a room full of experts suggested for her to look at a particular protein in her samples.  She had the audacity to tell us that her boss said if they are all saying to do this, then they all must be wrong!  Really?  You have a panel full of greybeards far more versed in this fi . . . More
Views: 2751 | Comments: 14
Last by Tideliar on Sep 29, 2010, 1:40pm
And this week another brave gladiator trudged from out of the mire to claim this week's win and that gladiator is Dr. Becca.  Enjoy your trophy.

Too much bourbon FTW!

And here is the rest of the field:

2.  Thomas Joseph (clawing his way up)

3.  Disgruntled Julie 

4.  PLS (stuck in 4th gear and 4th place)

5.  Chall (moving on up)

6.  Dark Overlord Brian Kreuger (strong showing after last week's win)

7.  Odyssey

8.  Jade Ed

9.   . . . More
Views: 185 | Comments: 0
In keeping with this week's bluegrass theme, here is "In Spite Of Ourselves" by John Prine and Iris DeMent.  A catchy little tune with funny lyrics.  Enjoy.



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Views: 486 | Comments: 3
Last by Genomic Repairman on Sep 28, 2010, 2:23pm
Hey guys,

so after last week's anger stroke almost took me out, I'm here to report back on what's shakin.  So in the past three weeks, I have have had to learn the background literature on an off-topic, come up with some hypotheses and experiments and prepare a rough draft of an F32 style postdoctoral fellowship proposal.  And I'm doing this while still holding down around 40 hours a week in the lab, to tell you this has been nothing short of excruciating is painful is being modest.  Now that I'm on the other side and have a week to revise and edit my proposal, its been a great exercise.  Eventually in your career you will have to come up with something new.  I found a gap in the science that looked interesting, cobbled together a hypothesis on what the heck is going on, and crafted together some reasonable experiments to test my hypothesis.  If this thing were like a movie, it would be as if a slasher film, sports flick, action movie, and softcore porn got together and had a baby.  Its got everything, mechanistic studies, mouse models, someone getting shot out of a cannon that is being circumnavigated by midgets wearing leotards and tutus while the William Tell Overture plays in the background.  But its not over t . . . More
Views: 203 | Comments: 0
This week I'm going with a Bluegrass theme and no better way to kick it off than with the live version Bron-Yr-Aur Stomp by Led Zeppelin (circa 1975).



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Views: 1330 | Comments: 21
Last by Genomic Repairman on Sep 25, 2010, 10:05am
Update:  Moonshine is the greatest attitude adjuster in the world. Swigging my peach flavored shine out on the balcony last night, my anger subsided and I came up with some great ideas for my exam.  However thanks to the hangover and the sparse amount of sleep, I'm feeling pretty haggard but motivated.

 

I'm sorry.  Really really sorry.  I lost my shit tonight.  I admit that the five minute long string of profanity and cursing heaven and earth, anyone responsible and their future progeny might have been a bit much.  But lets preface the events of my day:

-I have been at work for 17 hours.

-The only meal I have had in the last 30 hours has been a tiny sliver of cake.

-My booze stash has run dry.

-I had to teach a technician how to do western blots today at the end of the day.  He loaded the gel just fine, but he reversed the leads on the power source and didn't notice this until 45 minutes into the gel.  Thus setting my schedule back by more than hour after noticing and restarting the gel.

-Another Bio-Rad power pack failed.  I honestly felt sorry for you guys after my angry post, but not now.  Fuck you guys.  

-A beleaguered postdoc from another lab is intereste . . . More
Views: 258 | Comments: 0
"No Rain" by Blind Melon. RIP Shannon Hoon.



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Views: 328 | Comments: 2
Last by Genomic Repairman on Sep 23, 2010, 8:01am
"Bad Moon Rising" by Credence Clearwater Revival.  Enjoy



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Views: 448 | Comments: 0
Greedy Fly by Bush




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Views: 2257 | Comments: 40
Last by Brian Krueger, PhD on Oct 04, 2010, 10:58am
So the other day I was going through a field specific journal and found an article from a European group that was sort of interesting to me but outside of my field. I perused their data and was interested in their siRNA knockdown data and was reading further and decided to pull up a previous paper that they published back in 2008. Low and behold the siRNA data is their too! And its the same exact western blot looking at protein levels and loading control. I pulled the images and redacted the data to let you guys take a look at this.



They don't make mention that this is from the 2008 paper and try to pass it off as original data in the 2010 paper published in a different journal. I think that is the kicker too, I'm sure the original journal would be none to pleased that this EuroGroup was doing this. So what do you guys think, is this misconduct, is it okay? My own opinion is that this shit is pretty shady.

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Last by Suzy on Sep 22, 2010, 1:27pm
This week we crown a new champion and it is our very own Dark Overlord Brian Kreuger. So here is your trophy for you to proudly display in a celebration this week.



But don't get too careless Overlord as you barely eeked out the win since you were tied with Jade Ed who made a comeback to be #2 and the always elegant #3 GertyZ. Those of us mired within the fourth through eighth spots were: PLS,the ever lovable Thomas Joseph,Disgruntled Julie, Dr. O, and Odyssey. Those rooming at the clusterfuck also known as 9th through 13th were: Biochem Belle, . . . More
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Last by Genomic Repairman on Nov 01, 2010, 8:31am
Enjoy this groovy tunen from the '60's doo wop band from Brooklyn.

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Last by Richard Jones on Feb 18, 2011, 5:19pm
Today is not my day. Lets recount how bad today sucks.

-Still in the middle of a candidacy exam. The boss is hounding me for data and wondering why its taking so long. He seems not to be able to comprehend that I am dealing with the second biggest hurdle in my Ph.D. program. He nods and excoriation continues. Departmental and grad school policy dictates that we are allowed 50% time off for the exam yet this does not matter to my boss. He has a scientific boner for data and wants it now.


-Someone spilt soda all over my references for said exam and did not tell me. Thus ruining my stack of annotated journal articles and leaving me to reprint them again.

-Tried to run westerns but noob graduate student took my personal stash of gels and burned through them all last week. Using up my gradient gels to look for proteins that they could have easily poured 10% gels for if they weren't that lazy

-Got rejected for a fellowship award because "the award can be renewed for multiple years and your project is too far advanced. We prefer projects that much closer to initiating than those that are near completion." Wait a minute, I'm not graduating anytime soon? I guess I'm being penalized for being productive versus sitting around with my . . . More
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Last by Genomic Repairman on Sep 20, 2010, 3:40pm
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Last by Been There Bio Scam on Aug 10, 2012, 7:52pm
Dear Bio-Rad,
we were talking the other day in over in the Biotech Discussion Group about shameless product plugs and I made a remark that was slightly complimentary about one of your products. This felt weird and I caught (rightly so) an unmitigated amount of shit. Looking back on our relationship, lets recount the ways your product has failed me.

1. Over the past two years we have had three power packs fail.
2. Received a cracked gel box.
3. Actually gotten a polyacrylamide gel minus the fucking polyacrylamide in it.
4. Two of our thermocyclers have failed multiple times. One of them having the circuit board replaced twice and the heat block once. And they still freeze up.
5. The software interface for our ChemiDoc imaging system is perpetually mad at the hardware like it used to date and now it doesn't call anymore.
6. The BioLogic LP and BioFrac fraction collector. This thing broke on day one and had to be sent back and forth to the factory for service twice. And the BioFrac is a pissy little fucking ingrate that refuses to work. Luckily w . . . More
Views: 1090 | Comments: 9
Last by Evie on Sep 18, 2010, 1:07pm
Note: I don't work with stem cells, I have rudimentary knowledge but they lie outside my sphere of interest.

Usually every morning I read CNN to catch up on the news and this morning was no different. I happened upon an opinion article because I like to see zeitgeist is for different topics. I about had a fucking aneurysm this morning.

So Matt Bowman, a lawyer for the Alliance Defense Fund (not even giving these assholes the respect of proper hyperlink) just called stem cells outmoded in an CNN article. He is comparing ESCs to old cathode ray tube televisions and saying that now induced pluripotent stem cells (iPSCs) are the new HDTV. Lets note two things here, Matty Boy was a lawyer in the case that resulted in the stem cell research injunction and his organization is charged with the following mission:
“The Alliance Defense Fund is a legal alliance defending the right to hear and speak the Truth through strategy, training, funding, and litigation.”

Hang on guys, I have to shut off my crazy religious wackaloon alarms its going off at full alert. And I'm sure hard working ESC scientists are pleased when you make the blanket statement that, "ESC research is the $10,000 toilet," calling . . . More
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So its not too sciency, but its the 200th anniversary of Mexican Independence. So grab a Cerveza and shout out Cry of Dolores. And to help you celebrate this day, check out the video below. These guys are setting off what I would like to call fireworks but they really are explosives with sledgehammers. Apparently this guy thought he would be the show stopper and strapped an assload of explosive to his. We could have had a Darwin Award winner folks.

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Last by Evie on Sep 16, 2010, 12:44pm
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"The Shade of the Poison Tree" by Dashboard Confessional
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Last by Thomas Joseph on Sep 14, 2010, 3:07pm
"The Distance" by Cake
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Last by Genomic Repairman on Sep 14, 2010, 11:02pm
Necking out the gaggle of tie-breakers behind her to win the coveted award for week one is Lady Scientist. So here is your trophy for you to proudly display in a celebratory blog post.


Now lets go through the rest of the line up. 2nd through 7th place was decided by tie breakers and goes in the following order. Dr. O
DGT
GertyZ
LabSpaces resident thug blogger The Genomic Repairman
Biochem Belle
Dr. Becca.

One point behind this group were:
LabSpaces Overlord Brian
Chall
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"Diary of Jane" by Breaking Benjamin
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Last by Genomic Repairman on Sep 13, 2010, 1:56pm
So we all previously posted on our worklife balances and this is a sort of follow up for me that is me trying balance lab work and school obligations.

The goal of the exam is to crank out a postdoctoral grant proposal on an off-topic that our exam committee has selected. So I am currently a week or so into my 4 week exam. Last week was not a good week for me, I did not get nearly enough accomplished on the exam. During the exam process we are only allowed to work half time at most and the PI's are well aware of it too. But anyways lets review the week.



Monday:
I came in on the holiday and tried to do some writing and a little work that day but my boss showed up looking like he woke up in a ditch. I'm in the middle of a eight hour process of to make some complicated cell extracts and he wants to see some blots for a grant supplement. This supplement is important because if we get this grant it would fund my project and set our lab up in great shape as far as funding. So I was able to find some blots to please the boss, but I couldn't work them up until after my extract process was done. So instead of writing, I was scanning blots, making figures, and writing up the experimental details of each blot to give to my boss. So with what w . . . More
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Last by Evie on Sep 11, 2010, 9:50am
This post is for Evie in reference to our Hackers soundtrack discussion on my Orbital post. Enjoy.
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Last by Thomas Joseph on Sep 13, 2010, 8:56am
So here is a guest post from Thomas Joseph, a guy who I have been following for almost a year. He is insightful, got a solid message, and has left nothing but inspiring words of encouragement on my blog since I began trolling on his over a year ago. So give a big round of applause the guy!

Disclaimer: If you recognize yourself in this tale, it’s pure coincidence. Yes, the world of science is small, but not THAT small. And if I really am talking about you, don’t worry … I didn’t mention any names. Oh, and I may have embellished a tad in some spots, probably because James Frey was my ghost writer, and partly because it sounds a lot cooler that way. See what I did there? I embellished … James Frey wasn’t my ghost writer, just my muse.

If you’re like me, you cringe every time you hear someone say “Oh, this will be an easy pub!” Often it’s a graduate student caught up in the hype of their new project and enamored with the idea of the LPU (least publishable unit). These comments are also generally stated on the heels of said individual perusing some D-tier online-only, open access journal which has a total of two issues and will probably close before the end of the year … but whatevs . . . More
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Last by Professor in Training on Oct 02, 2010, 4:45pm
So the first set of winners, Jason Goldman and Professor in Training won on some hilarous comments about my gas line episode. So what did they win?

Well Jason won the following.

Jason's prize: A bottle of happy juice.


Since PiT isn't into alcohol I wanted to make sure I didn't short her on a prize so she got...



So I think I'll do a second round where I will announce the winner of funniest comment on my blog on Sept. 22. . . . More
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Enjoy Rod Stewart and Ronnie Wood at their finest.
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Last by Future Corpse on Sep 08, 2010, 4:55pm
So this is our theme post for the month of September and the topic was selected based upon polling the readers. Jokingly my first idea for the "I wish I knew.." was "I wish I knew my prom date was a filthy skank." So to shallowly delve into this story, lets take it back to high school days.


I wish I knew my prom date was a filthy skank
Sixteen year old Genomic Repairman was a nerdy kid who felt awkward in his own skin but was also somehow a two sport athlete (Baseball/Football). The prospects for me getting laid in high school were pretty much slim to fucking none as I was friends with many hot girls but could never seem to get out of the dreaded friend zone. So anywho not to beleaguer my pitiful adolescent love life, I ended up asking this gorgeous popular girl who was a fairly new student at our school to prom. There was no way she should have even considered my question. I mean this was like a 4 asking out a 9 on the dating scale, but she said yes. So fast forward to prom, I see my date hugging for just a little to long and chatting it updamn near every dude on the basketball and soccer team. Finally one of my platonic female friends who trapped me in the friend zone at an early age filled me in on the scoop. Apparently my prom date blew the basketball . . . More
Views: 324 | Comments: 2
Last by Evie on Sep 07, 2010, 11:56am
So exam committee selected one of my three proposed topics and communicated the choice to me one day after it should have been done. Thanks for nothing guys. The funny thing was, there was some heated voting on my topics and the one they chose ended up coming through some Byzantine BCS-style metric calculation. It was the first topic that I came up with and took the longest time to form so this will be interesting. The topic is based upon how a particular post-translation modification of a protein affects in role in a specific process in the cell. So I've already cooked up some easy in vitro rescue experiments in my mind, maybe a knock in to generate, etc. This should be fun, I think. I'll keep you guys updated.
TTFNMF . . . More
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for those of you not taking the day off...
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Last by Genomic Repairman on Sep 09, 2010, 8:00am
Many labs are either working with or have HEK293 cells frozen back in their repository. HEK293 embryonic kidney cells were cultured from an aborted fetus by Alex van der Eb, a researcher in Holland. Were you aware that they might not actually be of kidney cells and rather of neuronal origin. The cells also made Frank Graham, a researcher with van der Eb, famous for discovering that you could transfect some DNA if you just toss some calcium phosphate in with them. A scientist by the name of Gerry Shaw was trying to stain 293's with a monoclonal antibody that he created to beta-adrenergic receptor kinase 1 (beta-ARK) which should be expressed in the cells. Wanting to be a proper scientist, Shaw wanted to have a negative control for staining, so he grabbed a neurofilament antibody, which should not stain a kidney cell line right? WTF this and other neurofilament antibodies were able to stain the cells! Scratching your head right, so was Shaw. Using microarray data, it was shown that 293's expression pattern of neuronal genes similar to that of other neuronal cell lines. So if you are wanting an in vitro model for kidney research, you might want to go get another cell line.
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Last by unbalanced reaction on Sep 05, 2010, 11:54am
"The Small Print" by Muse.
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Last by Thomas Joseph on Sep 08, 2010, 10:34am
Hey guys I know we have a great community of readers here at LabSpaces and we are always trying to engage them. So I figure, why not have one of you guys do a guest post on the LabSpaces bad boy aka LabSpaces heartthrob Genomic Repairman's blog. So here is the deal if you want to do this, send me an email at genrepair@gmail.com and let me know what you are thinking of posting. I'll choose the most interesting post and put it up. But don't take this lightly, here are some of the requirements:
-Must be somewhat science related, doesn't have to be hardcore
-Funny is good, hilarious is better
-Profanity is welcomed if not encourage (I mean this is my blog after all)

Can't wait to see what filthy filth blog ideas come into the inbox.
TTFNMF . . . More
Views: 224 | Comments: 7
Last by Prabodh Kandala on Sep 03, 2010, 8:57am
Hey guys just as an update, Brian fixed the groups issue that we had where you could only see them if you were a registered user. Keeping with LabSpaces promise of full transparency, now everyone can see the groups section and look at the posts. Let me know if you have any problems.
TTFNMF . . . More
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Last by Lab Mom on Sep 02, 2010, 3:14pm
I'm sure those of you on campus have smelled it when passing down the hall or traipsing through your lab. It is a peculiar odor that seems to arise in the fall and takes months for the putrid stench to leave you nose. That's right its the stink of the FNG.

We just got a new crop of graduate students in. You know the ones who look like helpless tourists clutching to folders or Trapper Keepers of papers sticking out in all directions. Can you tell me where so and so's office is? Would you like to buy a pass to the pool on the roof? As senior graduate students we are unofficially tasked with being our brother's (and sister's) keeper. Its our job to take them under our wing and show them around, get them settled, help them with coursework if they struggle, and just generally keep them on the straight and narrow until they get in a permanent lab. Everyone of us has our own mentorship style. Some are out and out assholes, some are too nice, and some are the "absent father" in the model of mentorship. Where do I fall in this? Easy, right in the middle. My mentorship philosophy is a spoon full of sugar and a spoon full of dirt. If you screw up, I'm going to make fun of you, I can't help it, I have to. But after all the joking is o . . . More
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"Travelling Riverside" from Led Zeppelin's BBC sessions (I believe circa June 1969). Enjoy.
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Last by Suzy on Sep 02, 2010, 11:31am
Looking for group?

So I think an underutilized section of LabSpaces is the Groups section. Its a great way to foster interaction between users versus the static method whereby the post a comment to our blog and we post back to them. So I went ahead and set up two groups, Grad Students Section (a place for grasshoppers) and GenRep's Corner (a place for drunken debauchery and to hear me piss and moan about sports, politics, etc). This way I can post my science related stuff on the LabSpaces blog and keep my other stuff in the groups section because I don't like having a blog here and one at Blogger, I'd rather consolidate my stuff to one site. What do you guys think about the Groups section and how can we make it better. . . . More
Views: 342 | Comments: 9
Last by Genomic Repairman on Sep 03, 2010, 8:35am
So in the funniest post contest we actually had a tie. My favorite funniest contests belong to Jason Goldman and Professor in Training! So congratulations to you guys and get in touch with me so I can get those bottles of Jameson out to you both. And remember, I'll be naming another winner on September 7th so keep making fun of me. I mean it only cost me bottles of booze and years of therapy, but at least the shrink has a comfy couch. Come strong with the funnies and you can take a drink with me.

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Last by Genomic Repairman on Sep 02, 2010, 7:11pm

In college (for a short period of time) I had a job at a local biotechnology company that focused on vaccine research. The job was great at the time, I had pretty flexible hours and the pay was great for a college kid ($15/hour in 2003). The job was really gopher work but I got to go in and work 10pm-1am a couple of nights a week. My main responsibility was to make solutions, gels, change buffers on overnight dialysises, change antibody incubations on westerns, and make media for cell culture. My boss, a midlevel bench scientist was really happy with my work and since I worked fast, I usually had time at the end of my shift to do homework or study. Our company arranged to attend some unknown technology and jobs fair in Tampa, FL at the time. My boss invited another technician and I to go along for the weekend conference and not wanting to pass up a free all expenses paid vacation, I went. Apparently our company did not do the proper research ahead of time. We were signed up to be an exhibitor at a telecom conference and job fair. To put it politely, we were way the fuck out of place there. Our boss called the higher ups to explain the situation and was told to just grin and bear it through the weekend. So that Thursday evening we attended a banquet with telecom exe . . . More
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"Separate Ways" by Journey
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Last by Genomic Repairman on Sep 01, 2010, 12:39pm
So this week I have been emailing a former of mentor of mine just to keep in touch with them and heard some bad news. She was turned down for tenure at the SLAC (Small Liberal Arts College) where I did my undergrad. According to other sources that I have talked to, at her three year review they totally changed her requirements up on her. She was originally hired as for a chemical education TT job but was allowed to dabble in biochemistry as well. After her first year, the powers that be informed her that she could step up her biochemistry focus but maintain a baseline in the chem ed stuff. To her credit she did, coordinating labs for the introductory chem courses as well as teaching a course in science and math for teachers. However, when she hit her three year review, the T&P evaluation panel decided not to recognize her teaching and service in the biochemistry area. So this left a glaring hole in her tenure package and she had to scramble to try to get papers published and extra work done in the chemical education interest to satisfy this group. I was in a group of former students asked to evaluate and write a letter of reference for her tenure package. She came close to satisfying the requirements for tenure, but close only counts in horseshoes and hand grenades. Sad . . . More
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Last by Genomic Repairman on Aug 31, 2010, 9:42am
Warning by Incubus.
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Last by Genomic Repairman on Aug 31, 2010, 7:41pm
So I completely missed it and someone brought it up to me, that I've been blogging here for a little over a month. I guess Brian will give me a chip or something the next time the support group meets! Its been a fun month, at first it was a little hard to crank out regular content, but it has been a good exercise in writing and venting frustration. Lets see if I can make it another month here before they kick my sorry ass to the curb and replace me with a newer, sexier, more verbose model. Its been fun.
TTFNMF.
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Last by Genomic Repairman on Aug 30, 2010, 4:23pm
The New Yorker article focuses more on Collins and his background and religion and less on his dismay at the most recent ruling halting some funding for stem cell studies. Collins is even (purportedly) calling some PIs to let them know that their projects sadly won't be funded as a result of this. Check it out, pretty decent read.
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Last by Genomic Repairman on Sep 08, 2010, 9:52am
are playing a huge role (along with diversity initiatives) in getting women interested in science. I think its awesome that female scientists are posting about what they do, their successes, their hardships, and motivating a new generation of younger female scientists. So I just want to throw out a big high five to all you FemSciBloggers and here is a shortlist of the female science bloggers that I have been reading for a while:

Dr. Isis She a badass scientist not to mention a shoe connoisseur.
Candid Engineer Mango slicing made sexy baby.
The Unlikely Grad Student Great perspective from a mother in graduate school.
LabMom She'll riff on science as well as indulge you with the latest news about Big Brother.
Professor In Training An up and coming assistant professor and lover of Doritos.
Ambivalent Academic Fresh perspectiv . . . More
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Last by Lab Mom on Sep 01, 2010, 8:56pm


Hey guys,
I think my frequent postings are going to drop off for a little bit as unbeknownst to me my candidacy exam is going to start some time this week. Also I will not have the full amount of time to do it due to scheduling conflicts on the part of my exam committee. Am I worried? Yeah. Do I really care? Fuck no. I've come this far and I can do this shit. So until they pull the trigger on the starting pistol I'm going to be working like a mad man in the lab trying to get stuff done. Anyways I'll try to keep you guys updated on the process.
TTFNMF! (TaTa For Now MotherFuckers) . . . More
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Then enjoy "Can't Relate" by the Exies. I was actually turned on to this band back in 2004 when one of their other songs "Without" was featured on the video game MVP 04.
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Hey everyone I'm going to be traveling to Fort Worth, TX for a meeting in late October (23-26th). So if anyone in the general area wants to meet up for a drink, let me know and I'll buy the first round. . . . More
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Last by Genomic Repairman on Aug 27, 2010, 1:49pm
A favorite band of mine, even though the lead singer is a notorious asshole.
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Last by Dr. O on Aug 29, 2010, 6:07pm

So I am trying to organize an exam date for my qualifying exam but this task is quite arduous and damn near impossible. The ability to get five individuals into a room for an hour and a half or so seems to be a mystifying task and something on the order of some heroic quest. Seriously, one PI has like three days that entire month that is acceptable, another is pretty available, one has some scheduling issues, one hasn't responded, and the last is just dicking along until he tells me that he can do it. Seriously, I am scheduling way in advance and this is the fucking thanks I get. I've narrowed it down to two dates that should work but this is going to fuck me as I will do the exam either one or three days before I have to leave town for a conference and present a poster there. One exam committee member told me that the scheduling would be harder than the exam was and that if I survived it I would be fine for the exam. I'm starting to believe her. . . . More
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Last by Genomic Repairman on Aug 26, 2010, 10:52pm


I'm not currently on an animal protocol so why the fuck are you pestering me about animal manipulations in my lab. I already told you I am not working with mice. Fuck off man. Questions about arcane regulations and where I store protocols are not relevant to me dude. And don't get fucking pissy with me that I don't know where our signed copies of our animal protocols are at! I swear to fucking Aisha I will knock you in the jaw if you keep bothering me. Why can't you be like your other coworker and be polite and walk on and talk to someone else? Why me dude? Seriously, I'm starting to loose the will to live now that we are like ten minutes deep into this terrible never ending conversation. Hell have no fury like a woman scorned? Fuck that, hell have no fury like an annoying IACUC inspector. Goddamn I need a drink. . . . More
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Last by Thomas Joseph on Aug 27, 2010, 8:39am


So guys, gals, and guy/gals/pre-ops I am just trying to solicit some feedback about my blog here on LabSpaces. Do you guys want:
-more science articles from me kind of like yesterdays post on Fanconi Anemia and BRCA2.
-more science politics articles (trainee timelines for example).
-more/less calling out bullshit science.
-less ranting (tough shit, I'm always going to rant).
-keep/chunk the music section).

I treat my blog sort of like a business even though I receive absolutely no fucking compensation whatever. You the readers are in some form my customer and I'm want to improve your experience here. So tell me what you like, what you hate, etc. Also give me some feedback on any topic that you want to see me post on too. Thank you all for taking time out of your busy day to read my (some good, some bad, some libelous) musings and I greatly appreciate the feedback that you all have left me so far. Have a great day. . . . More
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Last by Genomic Repairman on Aug 26, 2010, 11:32am
Hey Oprah has a book club why can't I throw out some book suggestions. Some of these books are great for the lay reader, some of these are a little more in depth and some were pivotal to me studying science. So to begin with we'll kick off for the lay reader.


Surely Your Joking Mr. Feynman
This book is a classic, its really just a collection of anecdotal stories about Nobel Prize winner Richard Feynman's experience with science, going from early age to later on in life. Its written in a conversational style and feels as if Feynman is sitting across the table from you with a bongo recounting the hilarious stories of his life. Layman or science guru you gotta have this book.

Moving on to the biological realm, lets roll out with this next page-turner.


DNA: The Secret of Life
Written by another Nobel Prize winner James Watson, this book is great for any audience. Watson does a dynamite job of walking you through the advancement of the biological sciences, it success and its dismal f . . . More
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This is one of my favorite songs and it has special meaning to me this week as I had a friend in the Army return from his 6th deployment to the Middle East. So make sure to celebrate life and those around you today.
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Last by Tideliar on Aug 28, 2010, 7:30pm

I was working with someone else today rigging gas lines for an incubator when they did not properly clamp down the hose and it popped loose freely swinging around. All I saw was the hose pop, swing around, and then everything went black. From the other person's recount, the hose hit me in the testicle and I went down hard. And I guess I blacked out like a bitch. Now I'm at home icing Left Eye with a can of beer while posting this. I think I found my lab karma after watching the Epic Fail of yesterday. Let the jokes begin. . . . More
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Last by Genomic Repairman on Aug 25, 2010, 10:03am
Lately some groundbreaking work has been done in the DNA Repair field, specifically looking at the Homologous Recombination (HR) and Fanconi Anemia (FA) pathways. Two groups showed that FANCD2, a key protein in the FA pathway that is normally mono-ubiquitinated in response to damage, also has nuclease function and play a critical role in directing repair of double strand breaks (1,2). FA proteins push the cells towards the error-free HR versus non-homologous end joining (NHEJ) which can be error prone. So what does FANCD2 nuclease activity have to do with this? Well FANCD2 can apparently modify DNA ends leaving an unique 5’ overhang that is though not to be acceptable by FANCD1 aka, BRCA2. Well also recently a fungal ortholog of BRCA2 has been able to promote HR through the use of these ssDNA 5’ overhangs (3,4), so further work needs to be done to shore this up. And now it can, scientists at UC-Davis, were able to purify BRCA2. So I have to give a most sincere hat tip to these guys and all the other scientists working in this field that are chipping away at a more detailed mechanism into how these two pathways functions. So why should you even give a shit about t . . . More
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Last by Genomic Repairman on Aug 25, 2010, 9:39pm

Someone grabbed the wrong pestle that was too large for the dounce homogenizer and was trying to break open their cells when they jammed the too large glass pestle into the tube. Rather than trying to gently remove the pestle, they shoved it further down, blowing out the bottom of the glass tube and spilling their not really lysed cells onto the floor. I was making noises similar to a dolphin from laughing so hard and damn near blacked out due to lack of oxygen. I feel bad for the poor fucker for loosing something on the order of 10^9 cells but that was awesome.

I'm sure lab karma will eventually bite me on the ass for laughing at this.
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Asleep at the Wheel by The Wallflowers. Enjoy.
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Last by Tideliar on Aug 25, 2010, 2:00pm



The prize is the same but the way to win is I'll pick the funniest comment posted on my blog posts. Same announcement dates!

Update: Do to soul searching and mostly feedback I have decided to modify the contest so that it is the funniest comment post instead of negative points! This falls more in line with what is in the best interest of LabSpaces, my gracious host.
To begin with, I enjoy being annoying. And since the Dark Overlord is throwing a contest for the person with the most amount of points, I'm heading in the opposite direction with mine. I'm going to give a prize to the top three people with the least amount of points and I'm talking you gotta go negative folks. So I guess you better post some inflammatory comments and let the trolling begin. What's the prize you say? Will the Genomic Repairman reward with you notebooks that I have made doodles of unicorns in or Tshirts that I have already rubbed my balls on to be spiteful? Well maybe I would. But my prize is going to in the form of liquor. I'm going to offer up to you a liquor prize! The prize is a bottle of fine Jameson whiskey (a favorite of Dr. Isis or ComradePhysioProf). When will you pick the winner? I'm going to pick a winner on the morning . . . More
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Last by Nat on Sep 01, 2010, 2:58pm
I recently met with a faculty candidate who said that their PI has a finite limit on the years that you can postdoc in their lab. The candidate's PI instituted a rule that his postdoctoral fellows could only stay in his lab for a maximum of six years before they have to move on. And that means move on, no promoting your to some other title in the lab where you are still a glorified postdoc. Its 2185.6 days and you gotta get the fuck out. Kick rocks yo! I don't think this is an outlandish timeline but I imagine this has to apply pressure to the postdoc. To my knowledge and labs that I have been in and been around there is always a soft deadline but never a line in the sand put forth in writing at the outset. And this PI means business, even if you are productive when the clock strikes zero, here's a cardboard box, pack your shit. I'm too far away from this topic and have no clear opinion as to whether it is a good or bad policy. I can see the good in that you are getting folks to move on and not languish in one place for too long, I imagine the large percentage of those trainees might be moving on to second postdocs if they can't get a TT job lined up. What do you guys think?
So its tick tock you don't stop...
*This entry contains a Yo . . . More
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Last by becca on Aug 24, 2010, 8:38pm
happiness.
Our lab has a monthly ritual where we all take a break from practicing the dark arts for an hour or so to give the lab a good scrubbing down. I mean everything. We pull all of the glassware and chemicals out the cabinets to give them a good wipe down to remove any dust or spilled dirt. Centrifuges are cleaned and oiled. Rotors cleaned out. Benches wiped down. Hoods cleaned up and sanitized. We've got a bunch of nice new stuff, its gonna age but there is no reason that we shouldn't have nice old stuff too. Since we all pitch in together it maybe takes us an hour and a half each but represents over a full day of man hours worth of cleaning in a short concentrated time. And for all of us like many, we try to clean up behind ourselves but stuff falls through the cracks. This is our opportunity to to keep the place in tip top shape and the place is always clean to show off to visiting dignitaries. And for us, its a measure of pride, we do good science in a nice looking lab. What is your cleaning ritual? Oh and this is an interactive blog post so get off your collective asses and leave some comments. So scrub it down because we don't want no scrubs when it comes to cleaning.
*This entry contains a YouTube video* . . . More
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Feels like some Novocaine for the Soul?
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An opening salvo. Motherfucker we are in this together.

You realize the relationship between a core facility and its end users is tantamount to being in a boat. Your core is supported by a fucking huge NIH core grant and every time we publish a paper that your core is used we acknowledge your services and the core grant. This in turn makes it easier for you to renew the grant, keeping your sorry ass and you even harder working minions employed while subsidizing the cost of us doing science. Seems to work out well for everyone right? Your inflexibility to even want to change a protocol to better suit the outcomes of my experiment have drawn my ire. So instead of kowtowing to you, I'm taking my business else where. I'm going to use an outside vendor costing me 10-20% more to do the work and thus depriving you of the business because you are like some worn out floozy who can't seem to satisfy my needs. You claim to be so busy and that you guys do things the way you do that I did not think you would miss my business. I was wrong and I definitely did not expect you to raise a fucking stink about the whole issue that brought in many higher ups on this conference call that I alluded to in my earlier post today. Way to stack the deck against me asshole! S . . . More
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Last by Tideliar on Aug 24, 2010, 12:53pm
So today I have a conference call with a core facility about a project that I have currently running with them and a new one that I wish to initiate. Here is a run through of how conference calls in science work and probably every industry for that matter works. First off has anyone ever been on a conference call where anything useful happens? No! Every conference call that I have ever been on has worked like the following:
1. You call in. It takes 10 minutes to accomplish this even though you can just normally call anybody on the phone instantaneously or message them, conference calls take about 10 minutes to set up.
2. Somebody is always late.
3. So then you have the internal struggle of should we wait for this person or should we get started? Ok, lets just try doing random banter to kill time until they show up. Where are you guys from? Oh hows the weather in (city)? I heard about (insert random local celebrity)? You jive about random bullshit like that for another 10 minutes.
4. And then the person who was late will show up eventually after you have started. Which is a pain in the ass because you have to go back and rehash for that person what went on.
5. By now you are a half hour deep into the conference call and something comes up where some . . . More
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Last by Genomic Repairman on Aug 26, 2010, 11:44am
Enjoy Blue Monday by New Order or the cover by Orgy.


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Enjoy the folk tunes and hilarious lyrics of John Prine and Iris Demint and have a great weekend.
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Last by Genomic Repairman on Aug 20, 2010, 3:52pm
So until recently, recently being today, my father is the only person who could simultaneously give you a pat on the back and a kick in the ass. Well now my boss is the second guy to do this. We had a regular meeting to update him on the status of my projects, which has turned more from me listening to me talking and him listening to my strategies and giving me constructive criticism. Slowly I am making the evolutionary shift from LabTard to LabChimp. Yay opposable thumbs! My project has been a bit of a motherfucker to say the least. There is some knowledge of the protein that I study but no real definitive cellular function has been laid out. In fact there is not even a good antibody available for it. I burned through nearly six months on an antibody project (that I laboriously detailed over on my old blog at Blogger) that never worked. This go round has been very successful. He complemented me on my hard work and noted that all of my reagents are coming together and told me that I was in the driver's seat with this project. The cool thing is, I am picking up where a postdoc left off and it really is a kickass project. So after singing my praises he told me to hurry up with this goddamn antibody screening so I can get some real experiments done! If it ain' . . . More
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Last by Genomic Repairman on Aug 21, 2010, 12:00pm

I have two isogenic mouse embryonic fibroblast (MEF) cell lines that I want to use for colony formation assays and have tried to use in the past. One line is a wild type while the other is a knockout for my gene of interest and both lines are immortalized. My deficient cell line has a plating efficiency of around %50 but my wild type pitifully plates out at 6%. I've got through a couple of rounds to jump the plating efficiency up by plating small numbers of cells to select for cells that plate better. I'm running my first CFA with this subcloned population right now. I guess if this doesn't work maybe try a feeder layer? I don't want to do any experiments with them until I get comparable plating efficiencies. Got any feedback? I think if worse comes to worse, I'll have to get the mice back in (can get from repository) and make new MEF clones, which might not be such a bad thing. I'm trying to avoid the time and expense and just bootstrap this sucker. . . . More
Views: 126 | Comments: 6
Last by Genomic Repairman on Aug 19, 2010, 9:52am
Since our Audioslave music discussion yesterday stumbled onto Pearl Jam, I want to highlight the great solo music of Eddie Vedder.


And to finish it off, Pearl Jam's cover of the quintessential NYC punk rock band The Dead Boys "Sonic Reducer".
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Last by Genomic Repairman on Aug 21, 2010, 12:02pm
So I carpool to work with a technician in the lab that is adjacent to our space. This guy is relatively new having been here for a little over 7 months and is kind of hitting his stride. Watching him, he does a good job with his own work and pitches in to help others out. Right now he is covering his stuff as well as bailing out the project of a postdoc who is on maternity leave. This guy is slowly but steadily ascending the technician ladder to peak of technician Everest which is solidly held by Damn Good Technician. The only thing hampering this guy is a slacker postdoc who seems incapable of doing anything on his own and always needs someone to "show me how to do this really quickly." So Slacker Postdoc was getting ready to leave on vacation and two days before drops a note off on the desk of said technician that reads,
"Going on vacation in two days, taking tomorrow off as a personal day. Can you do these plasmid preps for me before I leave and submit for sequencing analysis. There are over 50 preps to do and I'm not sure of the numbering. If you have any questions, email me."

So with no prior notice, the Slacker Postdoc dropped 60 plasmid preps (and I'm not talking small scale) off to the semi-noob technician who is already valiantly trying to co . . . More
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Okay so lets run down the list of winners and who gets the credit...

1. DPM (Dr. Pussy Magnet) this goes to my old roommate Lump who was notorious on stiffing me for the power bill, this makes up for this buddy.
2. BHPS (BoHonkeyPimpStick) total credit goes to yours truly!
3. GRGLE (Genomic Repairman's Gift to Labs Everywhere) thanks to the excellent wordsmith Dr. Becca.
4. BASTARD (Best Antibody, Should Tag Antigen, Rabbit Derived) thumbs up at the awesome offering by Geeka.
5. FUFME (self-explanatory) me.
6. AIGWBFTS (All I Got Was A Buck For This Shit) random homeless guy I gave a buck to come up with a name.
7. GARJ (Get A Real Job) my dad.
8. DLTYF (Don't Listen To Your Father) my mother.
9. Caboose (an homage to a character in the popular machinima series Red vs Blue).

So the winner is (drumroll), my mom for DLTYF! I never have and I never will. So mom I know you don't drink so I'll be mailing you a gift certificate to your local Golden Corral so that you can enjoy an appetizer on my dime. Just kidding, do Golden Corrals still exist? I'll send you some flowers.

Honorable mention: The homeless guy who came up with AIGWBFTS, I'll duke you my spare change next time I see you. . . . More
Views: 497 | Comments: 3
Last by Rao on Sep 17, 2010, 2:37pm
So a recent presentation by a guy in the ncRNA field has captured my interest. He mainly talked about miRNAs and cancer and showed some serious evidence for the prominence of their involvement in certain leukemias. Another interesting facet of the field is that folks are starting to get into the business of looking at SNPs and seeing how those can effect miRNAs and other ncRNAs binding and effect on gene expression. A good offering of this are SNPs in genes that don't effect amino acid changes but that do effect cancer susceptibility, which is maybe changing the gene regulation by ncRNAs. Another question is are these things just fucking floating around in the cell or are they being targeted by chaperones or some other mechanisms? I find this field interesting, I don't really have any serious interest in this field nor does it impact my work directly right now, but I keep an eye on it. My only beef with the ncRNA people is that almost a third of the articles out there are reviews. Stop writing these fucking reviews and put the people back on the bench to learn more. And for that matter there are entirely too many review articles being written. In my field it seems as if there is a new review on Fanconi Anemia pathway coming out every other month, usually by the . . . More
Views: 156 | Comments: 5
Last by Genomic Repairman on Aug 18, 2010, 2:23pm
Enjoy Yesterday to Tomorrow from Audioslave (a mixing of Chris Cornell from Audioslave and the instrumental guys from Rage Against The Machine).
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Before I begin "Let Me Clear My Throat" (set to Fraggle Rock).


And to finish it off, you gotta do the "Humpty Dance"
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Last by Lab Mom on Aug 17, 2010, 8:54pm

So we are still taking folks for the challenge between the science bloggers here at LabSpaces and the folks at Scientopia and with a few other free agents thrown into the mix for flavor. So if you want to play in the Pick Em league (all you have to do is pick the damn winner, no wondering who to set as your wide out), shoot me a message so I can send out the invite emails and get this boondoggle of a competition off to a poor start. . . . More
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Last by Genomic Repairman on Aug 17, 2010, 2:10pm
So like many other folks I was suckered to sign up for LinkedIn, the the more professional business version of Facebook. While most of what I receive is utter bullshit, there are some good groups out there that I belong, specifically ones regarded to my line of work. However, my institutional affiliated groups are how can I put politely, horribly fucking shitty. For instance my undergrad alumni association allows non-alumni to join and peddle their fucking dross and post some of the most aggrandizing shit I have ever seen. If I get another post about how your webinar on social media marketing can take my business to the next level, I'm going to track you down and take a piss in your gas tank. Seriously, I'm not fucking kidding. But the professional groups are putting forward some good discussion and I think that is the only reason I even stick around this piss-poor excuse for a networking site. So I belong to the following groups:
-MolecularLab
-qPCR in Molecular Markers
-R4PN (Recombinant Protein Network)
-Antibody Network

So if you guys are members of any good groups on there, let us know with a comment. . . . More
Views: 160 | Comments: 1
Last by Tideliar on Aug 16, 2010, 1:12pm

Can't tell if this is an unfortunate looking woman or a terrible case of man boobs.
So if you haven't seen it floating around lately, first go read Professorlikesubstances's piece about the ethics of genomics data release and then the response by Mike the Mad Biologist. . . . More
Views: 658 | Comments: 5
Last by Genomic Repairman on Aug 16, 2010, 9:53am

So yesterday I did some extensive "field studies" of effective hydration with different samples (domestic, international, who knows whose fucking bathtub it came out of) on the aquatic landscape. And for all my hard work I've got some strange sunburns (right bicep, left forearm, etc). Got any remedies while you listen to Remedy by the Black Crowes.
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Views: 284 | Comments: 6
Last by MitoScientist on Aug 16, 2010, 4:43pm
What is the max number of cell lines that you actively work with at one time. I am now above ten and its getting a little hairy just from a management standpoint. So start posting comments. . . . More
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Last by Genomic Repairman on Aug 13, 2010, 4:48pm
You team-killing fucktard I want to shove you into a pond and hold you down until the bubbles stop coming up. Okay, that's a little harsh but lets rewind back to yesterday. We have designated workspace in our lab for radiation that also has a few common pieces of equipment nearby like heat blocks, minifuge, etc. So yesterday my kick ass lab manager was doing a regular inspection of the area and the Geiger counter sounded like it was having a fucking ecstasy-induced discotech seizure. Not that you had used radiation recently, it had been almost a week ago that you did. You claimed to have checked the work station to see if it was hot after you were finished and found nothing. I'm calling bullshit you dumpsterbaby, lets establish some facts, we don't magically get stuff radioactive after not working with it, the shit does not inverse decay fucktard. And more importantly you are the only person in the lab using radiation and you used it last week. And you dim-witted fucking slob. Normally I wouldn't give a shit that you contaminated that work station as I don't use it but you contaminated the minifuge and the heat blocks. And guess who uses those from time to time. Yeah folks this picture is not getting better. Come to find out my bench was hot s . . . More
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"Flathead" by the Fratellis. Have a good weekend.
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Last by biochem belle on Aug 15, 2010, 6:06am
So tomorrow is my apprentice, Genomic RepairGirl's last day. She will be giving her final presentation to all her fellow interns, faculty, trainees, and parents. I think her stuff is solid and she will give a great presentation if she can conquer her nerves. One on one she gives a solid walkthrough of the project but when in a group setting she trips up on her words and stammers. So I began making her present in front of larger groups to get her acclimated to speaking in front of bigger groups (n>1). Despite all of this I am very proud of what she has done. In ten short weeks she helped with two antibody screens learning how to do westerns on her own, cultured cell lines for me, isolated RNA and DNA, and understands the qPCR that we did on those samples, plus help out with making stable knockdown cell lines and doing some cloning. I gotta say I'm sad to see her go, she helped me get a ton of work done this summer. She may continue her internship in the fall hopefully because I could use some help. So its kind of fun to see them grow up from bewildered jackasses to semi-bewildered jackasses with marginal lab skills and shaky at best grasps on scientific concepts. So man if that is our future, at least they don't think humans rode dinosaurs like unicorns. . . . More
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or maybe its really the "Madison Blues" by George Thorogood & They Destroyers.
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