Brian Krueger is the owner, creator and coder of LabSpaces by night and Next Generation Sequencer by day. He is currently the Director of Genomic Analysis and Technical Operations for the Institute for Genomic Medicine at Columbia University Medical Center. In his blog you will find articles about technology, molecular biology, and editorial comments on the current state of science on the internet.
My posts are presented as opinion and commentary and do not represent the views of LabSpaces Productions, LLC, my employer, or my educational institution.
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How AAAS and Science magazine really feel about sexual harassment cases in science
Things have started to calm down a bit on my weekends so I've had more time to attend to fish. One of the crappiest things about owning a saltwater fish tank is that you can't just add fish to your system immediately. The fish come from the ocean covered in parasites, so you have to take extra special care of new additions to be sure they're clear of parasites and infections before you add them to your main "display" tank. Quarantining fish is absolutely a necessary evil. A lot of people neglect to do this, but it saves a ton of headaches. Trust me on this, you would much rather lose an $80 fish than get your $3000 display tank infested with a parasite!
Anyway, the fall got clogged with trips and holidays. Some of you may remember that back in October I tried to quarantine a yellow longnose butterfly fish and a powder blue tang that both died from a nasty bacterial infection. Given that quarantining fish takes at most 2 months if they get sick, I didn't have enough time to cycle in more fish before Turkey Day! Once Christmas passed, I was working on setting up an office tank for Whitney, so my quarantine system has been occupied with her inhabitants for the past two months... Finally I was able to pick up a new butterfly fish a few weeks ago. I treated him with formalin and malachite green to remove any external parasites. He ate and pooped well, with no apparent parasitic infections. I added him to the main tank yesterday and he seems to be getting along well with his new tank mates!
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I love that yellow-blue fish. Alhtough I thought at first it was a candy wrapper someone had droped in your tank!
Jaeson, that's not true at most places. Top tier, sure, but 1100+ should get you past the first filter of most PhD programs in the sciences. . . .Read More
All I can say is that GRE's really do matter at the University of California....I had amazing grades, as well as a Master's degree with stellar grades, government scholarships, publication, confere. . .Read More
Hi Brian, I am certainly interested in both continuity and accuracy of PacBio sequencing. However, I no longer fear the 15% error rate like I first did, because we have more-or-less worked . . .Read More
Great stuff Jeremy! You bring up good points about gaps and bioinformatics. Despite the advances in technology, there is a lot of extra work that goes into assembling a de novo genome on the ba. . .Read More
Brian,I don't know why shatz doesn't appear to be concerned about the accuracy of Pacbio for plant applications. You would have to ask him. We operate in different spaces- shatz is concerned a. . .Read More